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Plant Physiol, June 2001, Vol. 126, pp. 685-695
Pollen Germinates Precociously in the Anthers of
raring-to-go, an Arabidopsis Gametophytic
Mutant1
Sheila A.
Johnson and
Sheila
McCormick*
Plant Gene Expression Center, United States Department of
Agriculture/Agricultural Research Service, University of
California-Berkeley, 800 Buchanan Street, Albany, California
94710
Pollen hydration is usually tightly regulated and occurs in vivo
only when desiccated pollen grains acquire water from the female, thus
enabling pollen tube growth. Pollen tubes are easily visualized by
staining with decolorized aniline blue, a stain specific for callose.
We identified a mutant, raring-to-go, in which pollen
grains stained for callose before anther dehiscence. When
raring-to-go plants are transferred to high humidity,
pollen tubes dramatically elongate within the anther. As early as the bicellular stage, affected pollen grains in raring-to-go
plants acquire or retain water within the anther, and precociously
germinate. Thus, the requirement for contact with the female is
circumvented. We used pollen tetrad analysis to show that
raring-to-go is a gametophytic mutation, to our
knowledge the first gametophytic mutation in Arabidopsis that affects
early events in the pollination pathway. To aid in identifying
raring-to-go alleles, we devised a new technique for
screening pollen in bulk with decolorized aniline blue. We screened a
new M1 mutagenized population and identified several
additional mutants with a raring-to-go-like phenotype,
demonstrating the usefulness of this technique. Further, we isolated
other mutants (gift-wrapped pollen, polka dot
pollen, and emotionally fragile pollen) with
unexpected patterns of callose staining. We suggest that
raring-to-go and these other mutants may help dissect
components of the pathway that regulates pollen hydration and pollen
tube growth.
1
This work was supported by the U.S. Department
of Agriculture-Current Research Information System (grant no.
5335-21000-011-00D).
*
Corresponding author; e-mail sheilamc{at}nature.berkeley.edu; fax
510-559-5678.
© 2001 American Society of Plant Physiologists
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