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Plant Physiol, June 2001, Vol. 126, pp. 780-788
Ultraviolet B Radiation Enhances a Phytochrome-B-Mediated
Photomorphogenic Response in Arabidopsis1
Hernán E.
Boccalandro,
Carlos A.
Mazza,
M. Agustina
Mazzella,
Jorge J.
Casal, and
Carlos L.
Ballaré*
Instituto de Investigaciones Fisiológicas y Ecológicas
Vinculadas a la Agricultura, Consejo Nacional de Investigaciones
Científicas y Técnicas, Avenida San Martín
4453, C1417 DSE Buenos Aires, Argentina
Ultraviolet B radiation (UV-B, 290-315 nm) can cause damage and
induce photomorphogenic responses in plants. The mechanisms that
mediate the photomorphogenic effects of UV-B are unclear. In etiolated
Arabidopsis seedlings, a daily exposure to 2.5 h of UV-B enhanced
the cotyledon opening response induced by a subsequent red light (R)
pulse. An R pulse alone, 2.5 h of UV-B terminated with a far-red
pulse, or 2.5 h of continuous R caused very little cotyledon
opening. The enhancing effect of UV-B increased with fluence rate up to
approximately 7.58 µmol m 2 s1; at higher
fluence rates the response to UV-B was greatly reduced. The
phyA, phyA cry1, and cry1
cry2 mutants behaved like the wild type when exposed to UV-B
followed by an R pulse. In contrast, phyB, phyB
cry1, and phyB phyA mutants failed to open the
cotyledons. Thus, phytochrome B was required for the cotyledon opening
response to UV-B  R treatments, whereas phytochrome A and
cryptochromes 1 and 2 were not necessary under the conditions of our
experiments. The enhancing effect of low doses of UV-B on cotyledon
opening in uvr1 uvr2 and uvr1 uvr3
mutants, deficient in DNA repair, was similar to that found in the wild
type, suggesting that this effect of UV-B was not elicited by signals
derived from UV-B-induced DNA lesions (cyclobutane pyrimidine dimers
and 6-4 photoproducts). We conclude that low doses of UV-B, perceived
by a receptor system different from phytochromes, cryptochromes, or
DNA, enhance a de-etiolation response that is induced by active
phytochrome B.
1
This research was supported by grants from the
Secretariat of Science and Technology (Agencia Nacional de
Promoción Científica y Tecnológica, BID OC-AR802
PID no. 394 and PICT nos. 00342 and 05292).
*
Corresponding author; e-mail ballare{at}ifeva.edu.ar; fax 54-11-4514 8730.
© 2001 American Society of Plant Physiologists
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