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Plant Physiol, June 2001, Vol. 126, pp. 789-800
Comprehensive Expression Profile Analysis of the Arabidopsis
Hsp70 Gene Family1
Dong Yul
Sung,
Elizabeth
Vierling, and
Charles L.
Guy*
Plant Molecular and Cellular Biology Program, Department of
Environmental Horticulture, Institute of Food and Agricultural
Sciences, University of Florida, Gainesville, Florida 32611-0670
(D.Y.S., C.L.G.); and Department of Biochemistry and Molecular
Biophysics, University of Arizona, Tucson, Arizona 85721 (E.V.)
We isolated cDNA clones for two nuclear-encoded, organellar members
of the Arabidopsis hsp70 gene family, mtHsc70-2
(AF217458) and cpHsc70-2 (AF217459). Together with the
completion of the genome sequence, the hsp70 family in Arabidopsis
consists of 14 members unequally distributed among the five
chromosomes. To establish detailed expression data of this gene family,
a comprehensive reverse transcriptase-polymerase chain reaction
analysis for 11 hsp70s was conducted including analysis of
organ-specific and developmental expression and expression in response
to temperature extremes. All hsp70s showed 2- to 20-fold induction by
heat shock treatment except cpHsc70-1 and
mtHsc70-1, which were unchanged or repressed. The
expression profiles in response to low temperature treatment were more
diverse than those evoked by heat shock treatment. Both mitochondrial
and all cytosolic members of the family except Hsp70b
were strongly induced by low temperature, whereas endoplasmic reticulum
and chloroplast members were not induced or were slightly repressed.
Developmentally regulated expression of the heat-inducible Hsp70 in mature dry seed and roots in the absence of
temperature stress suggests prominent roles in seed maturation and root
growth for this member of the hsp70 family. This reverse
transcriptase-polymerase chain reaction analysis establishes the
complex differential expression pattern for the hsp70s in Arabidopsis
that portends specialized functions even among members localized to the
same subcellular compartment.
1
This work was supported by the Florida
Agricultural Experiment Station and University of Florida Plant
Molecular and Cellular Biology Program, by the U.S. Department of
Agriculture National Research Initiative (grant nos. 9800877 and
200000687 to C.L.G.), and by National Research Initiative funds and
State of Arizona Hatch funds (to E.V.). This research was approved for
publication as Florida Agricultural Experiment Station Journal Series
no. R-08014.
*
Corresponding author; e-mail clguy{at}ufl.edu; fax 352-392-3870.
© 2001 American Society of Plant Physiologists
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