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Plant Physiol, June 2001, Vol. 126, pp. 789-800

Comprehensive Expression Profile Analysis of the Arabidopsis Hsp70 Gene Family1

Dong Yul Sung, Elizabeth Vierling, and Charles L. Guy*

Plant Molecular and Cellular Biology Program, Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida 32611-0670 (D.Y.S., C.L.G.); and Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721 (E.V.)

We isolated cDNA clones for two nuclear-encoded, organellar members of the Arabidopsis hsp70 gene family, mtHsc70-2 (AF217458) and cpHsc70-2 (AF217459). Together with the completion of the genome sequence, the hsp70 family in Arabidopsis consists of 14 members unequally distributed among the five chromosomes. To establish detailed expression data of this gene family, a comprehensive reverse transcriptase-polymerase chain reaction analysis for 11 hsp70s was conducted including analysis of organ-specific and developmental expression and expression in response to temperature extremes. All hsp70s showed 2- to 20-fold induction by heat shock treatment except cpHsc70-1 and mtHsc70-1, which were unchanged or repressed. The expression profiles in response to low temperature treatment were more diverse than those evoked by heat shock treatment. Both mitochondrial and all cytosolic members of the family except Hsp70b were strongly induced by low temperature, whereas endoplasmic reticulum and chloroplast members were not induced or were slightly repressed. Developmentally regulated expression of the heat-inducible Hsp70 in mature dry seed and roots in the absence of temperature stress suggests prominent roles in seed maturation and root growth for this member of the hsp70 family. This reverse transcriptase-polymerase chain reaction analysis establishes the complex differential expression pattern for the hsp70s in Arabidopsis that portends specialized functions even among members localized to the same subcellular compartment.

1 This work was supported by the Florida Agricultural Experiment Station and University of Florida Plant Molecular and Cellular Biology Program, by the U.S. Department of Agriculture National Research Initiative (grant nos. 9800877 and 200000687 to C.L.G.), and by National Research Initiative funds and State of Arizona Hatch funds (to E.V.). This research was approved for publication as Florida Agricultural Experiment Station Journal Series no. R-08014.

* Corresponding author; e-mail clguy{at}ufl.edu; fax 352-392-3870.

© 2001 American Society of Plant Physiologists


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