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Plant Physiol, June 2001, Vol. 126, pp. 835-848
Proteomic Analysis of Arabidopsis Seed Germination and
Priming1
Karine
Gallardo,
Claudette
Job,
Steven P.C.
Groot,
Magda
Puype,
Hans
Demol,
Joël
Vandekerckhove, and
Dominique
Job*
Laboratoire Mixte Centre National de la Recherche
Scientifique-Institut National de la Recherche
Agronomique-Aventis, Aventis CropScience, Lyon, France (K.G.,
C.J., D.J.); Plant Research International, Wageningen, The Netherlands
(S.P.C.G.); and Flanders Interuniversity Institute for Biotechnology
and Department of Biochemistry, Gent University, Gent, Belgium (M.P.,
H.D., J.V.)
To better understand seed germination, a complex developmental
process, we developed a proteome analysis of the model plant Arabidopsis for which complete genome sequence is now available. Among
about 1,300 total seed proteins resolved in two-dimensional gels,
changes in the abundance (up- and down-regulation) of 74 proteins were
observed during germination sensu stricto (i.e. prior to radicle
emergence) and the radicle protrusion step. This approach was also used
to analyze protein changes occurring during industrial seed
pretreatments such as priming that accelerate seed germination and
improve seedling uniformity. Several proteins were identified by
matrix-assisted laser-desorption ionization time of flight mass
spectrometry. Some of them had previously been shown to play a role
during germination and/or priming in several plant species, a finding
that underlines the usefulness of using Arabidopsis as a model system
for molecular analysis of seed quality. Furthermore, the present study,
carried out at the protein level, validates previous results obtained
at the level of gene expression (e.g. from quantitation of
differentially expressed mRNAs or analyses of promoter/reporter
constructs). Finally, this approach revealed new proteins associated
with the different phases of seed germination and priming. Some of them are involved either in the imbibition process of the seeds (such as an
actin isoform or a WD-40 repeat protein) or in the seed dehydration
process (e.g. cytosolic glyceraldehyde-3-phosphate dehydrogenase).
These facts highlight the power of proteomics to unravel specific
features of complex developmental processes such as germination and to
detect protein markers that can be used to characterize seed vigor of
commercial seed lots and to develop and monitor priming treatments.
1
This work was supported by the European
Community (Fisheries, Agriculture and Agro-Industrial Research
project grant no. CT97-3711, "Genetic and Molecular Markers for Seed
Quality"), by the Region Rhône-Alpes (Programme
"Biotechnologies: La Semence"), and by the Fund for Scientific
Research of Flanders.
*
Corresponding author; e-mail dominique.job{at}aventis.com; fax
33-4-72-85-22-97.
© 2001 American Society of Plant Physiologists
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