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Plant Physiol, June 2001, Vol. 126, pp. 849-860
Jasmonate-Dependent Induction of Indole Glucosinolates in
Arabidopsis by Culture Filtrates of the Nonspecific Pathogen
Erwinia carotovora1
Günter
Brader,
Éva
Tas, and
E. Tapio
Palva*
Department of Biosciences (G.B., E.T., E.T.P.) and Division of
Genetics and Institute of Biotechnology (E.T.P.), University of
Helsinki, POB 56, FIN-00014 Helsinki, Finland
Elicitors from the plant pathogen Erwinia carotovora
trigger coordinate induction of the tryptophan (Trp) biosynthesis
pathway and Trp oxidizing genes in Arabidopsis. To elucidate the
biological role of such pathogen-induced activation we characterized
the production of secondary defense metabolites such as camalexin and
indole glucosinolates derived from precursors of this pathway. Elicitor
induction was followed by a specific increase in
3-indolylmethylglucosinolate (IGS) content, but only a barely
detectable accumulation of the indole-derived phytoalexin camalexin.
The response is mediated by jasmonic acid as shown by lack of IGS
induction in the jasmonate-insensitive mutant coi1-1. In
accordance with this, methyl jasmonate was able to trigger IGS
accumulation in Arabidopsis. In contrast, ethylene and salicylic acid
seem to play a minor role in the response. They did not trigger
alterations in IGS levels, and methyl jasmonate- or elicitor-induced
IGS accumulation in NahG and ethylene-insensitive ein2-1 mutant plants was similar as in the wild type.
The breakdown products of IGS and other glucosinolates were able to
inhibit growth of E. carotovora. The results suggest
that IGS is of importance in the defense against bacterial pathogens.
1
This work was supported by the Academy of
Finland (Finnish Centre of Excellence program), Biocentrum Helsinki,
and the European Union (contract no. ERBIC15-CT96-0908).
*
Corresponding author; e-mail tapio.palva{at}helsinki.fi; fax
358-9-191-59076.
© 2001 American Society of Plant Physiologists
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