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Plant Physiol, June 2001, Vol. 126, pp. 861-874
Seed-Specific Over-Expression of an Arabidopsis cDNA Encoding a
Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed
Weight1
Colette
Jako,
Arvind
Kumar,
Yangdou
Wei,
Jitao
Zou,
Dennis L.
Barton,
E. Michael
Giblin,
Patrick S.
Covello, and
David C.
Taylor*
Seed Oil Biotechnology Group, National Research Council of Canada,
Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon,
Saskatchewan S7N 0W9, Canada
We recently reported the cloning and characterization of an
Arabidopsis (ecotype Columbia) diacylglycerol acyltransferase cDNA (Zou
et al., 1999) and found that in Arabidopsis mutant line AS11, an ethyl
methanesulfonate-induced mutation at a locus on chromosome II
designated as Tag1 consists of a 147-bp insertion in the
DNA, which results in a repeat of the 81-bp exon 2 in the Tag1 cDNA. This insertion mutation is correlated with an
altered seed fatty acid composition, reduced diacylglycerol
acyltransferase (DGAT; EC 2.3.1.20) activity, reduced seed
triacylglycerol content, and delayed seed development in the AS11
mutant. The effect of the insertion mutation on microsomal
acyl-coenzyme A-dependent DGAT is examined with respect to DGAT
activity and its substrate specificity in the AS11 mutant relative to
wild type. We demonstrate that transformation of mutant AS11 with a
single copy of the wild-type Tag1 DGAT cDNA can
complement the fatty acid and reduced oil phenotype of mutant AS11.
More importantly, we show for the first time that seed-specific
over-expression of the DGAT cDNA in wild-type Arabidopsis enhances oil
deposition and average seed weight, which are correlated with DGAT
transcript levels. The DGAT activity in developing seed of transgenic
lines was enhanced by 10% to 70%. Thus, the current study confirms
the important role of DGAT in regulating the quantity of seed
triacylglycerols and the sink size in developing seeds.
1
This work was supported by the Saskatchewan
Department of Agriculture and Food (grant nos. 96000046 and 19990362).
This is National Research Council of Canada publication no. 43794.
*
Corresponding author; e-mail david.taylor{at}nrc.ca; fax
306-975-4839.
© 2001 American Society of Plant Physiologists
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