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Plant Physiol, June 2001, Vol. 126, pp. 883-889
Induction of ApL3 Expression by Trehalose
Complements the Starch-Deficient Arabidopsis Mutant
adg2-1 Lacking ApL1, the Large Subunit of ADP-Glucose
Pyrophosphorylase1
Thorsten
Fritzius,
Roger
Aeschbacher,2
Andres
Wiemken, and
Astrid
Wingler3*
Botanisches Institut, Universität Basel, Hebelstrasse 1, CH-4056 Basel, Switzerland
The disaccharide trehalose has strong effects on plant metabolism
and development. In Arabidopsis seedlings, growth on
trehalose-containing medium leads to an inhibition of root elongation,
an accumulation of starch in the shoots, an increased activity of
ADP-Glc pyrophosphorylase (AGPase), and an induction of the expression
of the AGPase gene, ApL3 (A. Wingler, T. Fritzius, A. Wiemken, T. Boller, R.A. Aeschbacher [2000] Plant Physiol 124:
105-114). We used Arabidopsis mutants deficient in starch synthesis to
examine whether the primary effect of trehalose was to affect
carbohydrate allocation by the induction of AGPase in the
photosynthetic tissue. In a mutant lacking the large AGPase subunit,
ApL1, (adg2-1 mutant) growth on trehalose restored
AGPase activity and led to a strong accumulation of starch in the
shoots. In contrast, starch synthesis could not be induced in a mutant
lacking the small AGPase subunit, ApS, (adg1-1 mutant) or in a mutant lacking plastidic phosphoglucomutase
(pgm1-1 mutant). These results indicate that ApL3 can
substitute for ApL1 in the AGPase complex. In addition, root elongation
in the mutants, especially in the adg1-1 mutant, was
partially resistant to trehalose, suggesting that the induction of
ApL3 expression and the resulting accumulation of starch
in the shoots were partially responsible for the effects of trehalose
on the growth of wild-type plants.
1
This work was supported by the Swiss National
Science Foundation (grant no. 3100-042535.94 to A.W.) and by Novartis
Agribusiness Biotechnology Research, Inc.
2
Present address: Botanisches Institut, Universität
Basel, Schönbeinstrasse 6, CH-4056 Basel, Switzerland.
3
Present address: Department of Biology, University
College London, Gower Street, London WC1E 6BT, UK.
*
Corresponding author; e-mail a.wingler{at}ucl.ac.uk; fax
44-20-7679-7096.
© 2001 American Society of Plant Physiologists
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