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Plant Physiol, July 2001, Vol. 126, pp. 1024-1030
Evidence for a Role of Salicylic Acid in the Oxidative Damage
Generated by NaCl and Osmotic Stress in Arabidopsis
Seedlings1
Omar
Borsani,
Victoriano
Valpuesta, and
Miguel A.
Botella*
Departamento de Biología Molecular y Bioquímica,
Universidad de Málaga, 29071 Málaga, Spain
Previous studies have shown that salicylic acid (SA) is an
essential component of the plant resistance to pathogens. We now show
that SA plays a role in the plant response to adverse environmental conditions, such as salt and osmotic stresses. We have studied the
responses of wild-type Arabidopsis and an SA-deficient transgenic line
expressing a salicylate hydroxylase (NahG) gene to
different abiotic stress conditions. Wild-type plants germinated under
moderate light conditions in media supplemented with 100 mM
NaCl or 270 mM mannitol showed extensive necrosis in the
shoot. In contrast, NahG plants germinated under the
same conditions remained green and developed true leaves. The lack of
necrosis observed in NahG seedlings under the same
conditions suggests that SA potentiates the generation of reactive
oxygen species in photosynthetic tissues during salt and osmotic
stresses. This hypothesis is supported by the following observations.
First, the herbicide methyl viologen, a generator of superoxide radical
during photosynthesis, produced a necrotic phenotype only in wild-type
plants. Second, the presence of reactive oxygen-scavenging compounds in
the germination media reversed the wild-type necrotic phenotype seen
under salt and osmotic stress. Third, a greater increase in the
oxidized state of the glutathione pool under NaCl stress was observed
in wild-type seedlings compared with NahG seedlings.
Fourth, greater oxidative damage occurred in wild-type seedlings
compared with NahG seedlings under NaCl stress as
measured by lipid peroxidation. Our data support a model for SA
potentiating the stress response of the germinating Arabidopsis seedling.
1
This work was supported by the Universidad de
Málaga, Junta de Andalucía (grant no. AGR-168) and by
the European Union (return grant to M.A.B.).
*
Corresponding author; e-mail mabotella{at}uma.es; fax
34-952-131932.
© 2001 American Society of Plant Physiologists
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