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Plant Physiol, July 2001, Vol. 126, pp. 1042-1054
Enhanced Accumulation of BiP in Transgenic Plants Confers
Tolerance to Water Stress1
Fátima C.
Alvim,
Sônia M.B.
Carolino,
Júlio C.M.
Cascardo,2
Cristiano C.
Nunes,
Carlos A.
Martinez,
Wagner C.
Otoni, and
Elizabeth P.B.
Fontes*
Departamento de Biologia Vegetal (F.C.A., S.M.B.C., J.C.M.C.,
C.A.M., W.C.O.) and Departamento de Bioquímica e Biologia
Molecular (C.C.N., E.P.B.F.), BIOAGRO-Universidade Federal de
Viçosa, 36571.000 Viçosa, Minas Gerais, Brazil
The binding protein (BiP) is an important component of endoplasmic
reticulum stress response of cells. Despite extensive studies in
cultured cells, a protective function of BiP against stress has not yet
been demonstrated in whole multicellular organisms. Here, we have
obtained transgenic tobacco (Nicotiana tabacum L. cv
Havana) plants constitutively expressing elevated levels of BiP or its
antisense cDNA to analyze the protective role of this endoplasmic
reticulum lumenal stress protein at the whole plant level. Elevated
levels of BiP in transgenic sense lines conferred tolerance to the
glycosylation inhibitor tunicamycin during germination and tolerance to
water deficit during plant growth. Under progressive drought, the leaf
BiP levels correlated with the maintenance of the shoot turgidity and
water content. The protective effect of BiP overexpression against
water stress was disrupted by expression of an antisense BiP cDNA
construct. Although overexpression of BiP prevented cellular
dehydration, the stomatal conductance and transpiration rate in
droughted sense leaves were higher than in control and antisense
leaves. The rate of photosynthesis under water deficit might have
caused a degree of greater osmotic adjustment in sense leaves because
it remained unaffected during water deprivation, which was in
marked contrast with the severe drought-induced decrease in the
CO2 assimilation in control and antisense leaves. In
antisense plants, the water stress stimulation of the antioxidative
defenses was higher than in control plants, whereas in droughted
sense leaves an induction of superoxide dismutase activity was not
observed. These results suggest that overexpression of BiP in plants
may prevent endogenous oxidative stress.
1
This research was supported by the
Brazilian government agencies Financiadora de Estudas e Projetos (grant
no. 64.94.0113.00 to E.P.B.F.), Programa de Apoio e Desenvolvimento
Científico e Tecnológico (grant no. 62.0272/97.0 to E.P.B.F.), and
Fundação de Amparo à Pesquisa do Estado de Minas
Gerais (grant no. CBB 2598/98 to E.P.B.F.). F.C.A. was supported by a
Fundação de Amparo à Pesquisa do Estado de Minas Gerais graduate
fellowship from the Minas Gerais State (Brazil). S.M.B.C. and J.C.M.C.
received Conselho Nacional de Desenvolvimento Científico e
Tecnológico graduate fellowships from the Brazilian Government.
2
Present address: Departamento de Ciências
Biológicas-Universidade Estodual de Santa Cruz, Rodovia
Itabuna-Ilhéus, Km 16, 45650.00 Ilhéus, Bahia, Brazil.
*
Corresponding author; e-mail bbfontes{at}mail.ufv.br; fax:
55-31-3899-2864.
© 2001 American Society of Plant Physiologists
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