|
Plant Physiol, July 2001, Vol. 126, pp. 1072-1084
Differential Expression of Members of the Annexin Multigene
Family in Arabidopsis1
Greg B.
Clark,
Allen
Sessions,
Dennis J.
Eastburn, and
Stanley J.
Roux*
Department of Molecular Cell and Developmental Biology, University
of Texas, Austin, Texas 78713 (G.B.C., D.E., S.J.R.); and
Department of Biology, University of California at San Diego, La
Jolla, California 92093 (A.S.)
Although in most plant species no more than two annexin genes have
been reported to date, seven annexin homologs have been identified in
Arabidopsis, Annexin Arabidopsis 1-7 (AnnAt1-AnnAt7). This establishes that annexins can be a diverse, multigene protein family in a single plant species. Here we compare and analyze these
seven annexin gene sequences and present the in situ RNA localization
patterns of two of these genes, AnnAt1 and
AnnAt2, during different stages of Arabidopsis
development. Sequence analysis of AnnAt1-AnnAt7 reveals
that they contain the characteristic four structural repeats including
the more highly conserved 17-amino acid endonexin fold region found in
vertebrate annexins. Alignment comparisons show that there are
differences within the repeat regions that may have functional
importance. To assess the relative level of expression in various
tissues, reverse transcription-PCR was carried out using
gene-specific primers for each of the Arabidopsis annexin genes. In
addition, northern blot analysis using gene-specific probes indicates
differences in AnnAt1 and AnnAt2
expression levels in different tissues. AnnAt1 is
expressed in all tissues examined and is most abundant in stems,
whereas AnnAt2 is expressed mainly in root tissue and to
a lesser extent in stems and flowers. In situ RNA localization
demonstrates that these two annexin genes display developmentally
regulated tissue-specific and cell-specific expression patterns. These
patterns are both distinct and overlapping. The developmental
expression patterns for both annexins provide further support for the
hypothesis that annexins are involved in the Golgi-mediated secretion
of polysaccharides.
1
This work was supported by the National
Aeronautics and Space Administration (grant no. NAGW 1519 to S.J.R.).
A.S. was funded by a Department of Energy fellowship from the Life
Sciences Research Foundation.
*
Corresponding author; e-mail sroux{at}uts.cc.utexas.edu; fax
512-232-3402.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
N. Patel, N. Hamamouch, C. Li, T. Hewezi, R. S. Hussey, T. J. Baum, M. G. Mitchum, and E. L. Davis
A nematode effector protein similar to annexins in host plants
J. Exp. Bot.,
November 3, 2009;
(2009)
erp293v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Konopka-Postupolska, G. Clark, G. Goch, J. Debski, K. Floras, A. Cantero, B. Fijolek, S. Roux, and J. Hennig
The Role of Annexin 1 in Drought Stress in Arabidopsis
Plant Physiology,
July 1, 2009;
150(3):
1394 - 1410.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Laohavisit, J. C. Mortimer, V. Demidchik, K. M. Coxon, M. A. Stancombe, N. Macpherson, C. Brownlee, A. Hofmann, A. A.R. Webb, H. Miedema, et al.
Zea mays Annexins Modulate Cytosolic Free Ca2+ and Generate a Ca2+-Permeable Conductance
PLANT CELL,
February 1, 2009;
21(2):
479 - 493.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N.-J. Hu, A. M. Yusof, A. Winter, A. Osman, A. K. Reeve, and A. Hofmann
The Crystal Structure of Calcium-bound Annexin Gh1 from Gossypium hirsutum and Its Implications for Membrane Binding Mechanisms of Plant Annexins
J. Biol. Chem.,
June 27, 2008;
283(26):
18314 - 18322.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Mortimer, A. Laohavisit, N. Macpherson, A. Webb, C. Brownlee, N. H. Battey, and J. M. Davies
Annexins: multifunctional components of growth and adaptation
J. Exp. Bot.,
February 10, 2008;
(2008)
erm344v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. M. Gorecka, C. Thouverey, R. Buchet, and S. Pikula
Potential Role of Annexin AnnAt1 from Arabidopsis thaliana in pH-Mediated Cellular Response to Environmental Stimuli
Plant Cell Physiol.,
June 1, 2007;
48(6):
792 - 803.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Ide, N. Nagasaki, R. Tomioka, M. Suito, T. Kamiya, and M. Maeshima
Molecular properties of a novel, hydrophilic cation-binding protein associated with the plasma membrane
J. Exp. Bot.,
March 1, 2007;
58(5):
1173 - 1183.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Faurobert, C. Mihr, N. Bertin, T. Pawlowski, L. Negroni, N. Sommerer, and M. Causse
Major Proteome Variations Associated with Cherry Tomato Pericarp Development and Ripening
Plant Physiology,
March 1, 2007;
143(3):
1327 - 1346.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Ide, R. Tomioka, Y. Ouchi, T. Kamiya, and M. Maeshima
Transcriptional Induction of Two Genes for CCaPs, Novel Cytosolic Proteins, in Arabidopsis thaliana in the Dark
Plant Cell Physiol.,
January 1, 2007;
48(1):
54 - 65.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kiba, T. Naitou, N. Koizumi, T. Yamashino, H. Sakakibara, and T. Mizuno
Combinatorial Microarray Analysis Revealing Arabidopsis Genes Implicated in Cytokinin Responses through the His->Asp Phosphorelay Circuitry
Plant Cell Physiol.,
February 1, 2005;
46(2):
339 - 355.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Okamoto, K. Higuchi, T. Shinkawa, T. Isobe, H. Lorz, T. Koshiba, and E. Kranz
Identification of Major Proteins in Maize Egg Cells
Plant Cell Physiol.,
October 15, 2004;
45(10):
1406 - 1412.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Lee, E. J. Lee, E. J. Yang, J. E. Lee, A. R. Park, W. H. Song, and O. K. Park
Proteomic Identification of Annexins, Calcium-Dependent Membrane Binding Proteins That Mediate Osmotic Stress and Abscisic Acid Signal Transduction in Arabidopsis
PLANT CELL,
June 1, 2004;
16(6):
1378 - 1391.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Samaj, F. Baluska, and H. Hirt
From signal to cell polarity: mitogen-activated protein kinases as sensors and effectors of cytoskeleton dynamicity
J. Exp. Bot.,
January 2, 2004;
55(395):
189 - 198.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
