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Plant Physiol, July 2001, Vol. 126, pp. 1072-1084
Differential Expression of Members of the Annexin Multigene
Family in Arabidopsis1
Greg B.
Clark,
Allen
Sessions,
Dennis J.
Eastburn, and
Stanley J.
Roux*
Department of Molecular Cell and Developmental Biology, University
of Texas, Austin, Texas 78713 (G.B.C., D.E., S.J.R.); and
Department of Biology, University of California at San Diego, La
Jolla, California 92093 (A.S.)
Although in most plant species no more than two annexin genes have
been reported to date, seven annexin homologs have been identified in
Arabidopsis, Annexin Arabidopsis 1-7 (AnnAt1-AnnAt7). This establishes that annexins can be a diverse, multigene protein family in a single plant species. Here we compare and analyze these
seven annexin gene sequences and present the in situ RNA localization
patterns of two of these genes, AnnAt1 and
AnnAt2, during different stages of Arabidopsis
development. Sequence analysis of AnnAt1-AnnAt7 reveals
that they contain the characteristic four structural repeats including
the more highly conserved 17-amino acid endonexin fold region found in
vertebrate annexins. Alignment comparisons show that there are
differences within the repeat regions that may have functional
importance. To assess the relative level of expression in various
tissues, reverse transcription-PCR was carried out using
gene-specific primers for each of the Arabidopsis annexin genes. In
addition, northern blot analysis using gene-specific probes indicates
differences in AnnAt1 and AnnAt2
expression levels in different tissues. AnnAt1 is
expressed in all tissues examined and is most abundant in stems,
whereas AnnAt2 is expressed mainly in root tissue and to
a lesser extent in stems and flowers. In situ RNA localization
demonstrates that these two annexin genes display developmentally
regulated tissue-specific and cell-specific expression patterns. These
patterns are both distinct and overlapping. The developmental
expression patterns for both annexins provide further support for the
hypothesis that annexins are involved in the Golgi-mediated secretion
of polysaccharides.
1
This work was supported by the National
Aeronautics and Space Administration (grant no. NAGW 1519 to S.J.R.).
A.S. was funded by a Department of Energy fellowship from the Life
Sciences Research Foundation.
*
Corresponding author; e-mail sroux{at}uts.cc.utexas.edu; fax
512-232-3402.
© 2001 American Society of Plant Physiologists
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