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Plant Physiol, July 2001, Vol. 126, pp. 1105-1115
An Arabidopsis Mutant Tolerant to Lethal Ultraviolet-B Levels
Shows Constitutively Elevated Accumulation of Flavonoids and Other
Phenolics1
Kim
Bieza2 and
Rodrigo
Lois*
Department of Biological Science, California State University, 800 North State College Boulevard, Fullerton, California 92834
The isolation and characterization of mutants hypersensitive to
ultraviolet (UV) radiation has been a powerful tool to learn about the
mechanisms that protect plants against UV-induced damage. To increase
our understanding of the various mechanisms of defense against UVB
radiation, we searched for mutations that would increase the level of
tolerance of Arabidopsis plants to UV radiation. We describe a single
gene dominant mutation (uvt1) that leads to a remarkable
tolerance to UVB radiation conditions that would kill wild-type plants.
Pigment analyses show a constitutive increase in accumulation of
UV-absorbing compounds in uvt1 that increases the
capacity of the leaves to block UVB radiation and therefore is likely
to be responsible for the elevated resistance of this mutant to UVB
radiation. These increases in absorption in the UV region are due, at
least in part, to increases in flavonoid and sinapate accumulation.
Expression of chalcone synthase (CHS) mRNA was shown to be
constitutively elevated in uvt1 plants, suggesting that
the increases in absorption may be a consequence of changes in gene
expression. Expression of CHS in uvt1 was shown to be still inducible
by UV, indicating that the uvt1 lesion may not affect the UV-mediated
regulation of CHS gene expression. Our data support an important role
for UV screens in the overall protection of plants to UVB radiation.
The uvt1 mutant could prove to be an important tool to
elucidate further the exact role of UV-absorbing pigments in UV
protection as well as the relative contribution of other mechanisms to
the overall tolerance of plants to UV radiation.
1
This work was supported by the National
Institute of Environmental Health Sciences, National Institutes of
Health (grant no. R29-ES07575). Its contents are solely the
responsibility of the authors and do not necessarily represent the
official views of the National Institute of Environmental Health
Sciences, National Institutes of Health. This work was also supported
by the California State University Special Fund for Research
Scholarship and Creative Activity.
2
Present Address: The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037.
*
Corresponding author; e-mail rlois{at}fullerton.edu; fax
714-278-5044.
© 2001 American Society of Plant Physiologists
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