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Plant Physiol, July 2001, Vol. 126, pp. 1139-1149
NAD Malic Enzyme and the Control of Carbohydrate Metabolism in
Potato Tubers1
Helen L.
Jenner,2
Brenda M.
Winning,
A. Harvey
Millar,3
Kim L.
Tomlinson,
Christopher J.
Leaver, and
Steven A.
Hill*
Department of Plant Sciences, University of Oxford, South Parks
Road, Oxford OX1 3RB, United Kingdom
Potato (Solanum tuberosum) plants were transformed
with a cDNA encoding the 59-kD subunit of the potato tuber
NAD-dependent malic enzyme (NADME) in the antisense orientation.
Measurements of the maximum catalytic activity of NADME in tubers
revealed a range of reductions in the activity of this enzyme down to
40% of wild-type activity. There were no detrimental effects on plant growth or tuber yield. Biochemical analyses of developing tubers indicated that a reduction in NADME activity had no detectable effects
on flux through the tricarboxylic acid cycle. However, there was
an effect on glycolytic metabolism with significant increases in the
concentration of 3-phosphoglycerate and
phosphoenolpyruvate. These results suggest that
alterations in the levels of intermediates toward the end of the
glycolytic pathway may allow respiratory flux to continue at wild-type
rates despite the reduction in NADME. There was also a statistically
significant negative correlation between NADME activity and tuber
starch content, with tubers containing reduced NADME having an
increased starch content. The effect on plastid metabolism may result
from the observed glycolytic perturbations.
1
This work was supported by the Biotechnology and
Biological Sciences Research Council of the UK (Biochemistry of
Metabolic Regulation in Plants grant to C.J.L. and studentship
to H.L.J.), by the Human Frontiers Science Programme (fellowship to
A.H.M.), and by Zeneca Agrochemicals (grant to C.J.L. and
S.A.H.).
2
Present address: Department of Applied Genetics, John
Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
3
Present address: Department of Biochemistry, University
of Western Australia, Nedlands, WA 6907, Australia.
*
Corresponding author; e-mail steven.hill{at}plants.ox.ac.uk;
fax 44-1865-275074.
© 2001 American Society of Plant Physiologists
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