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Plant Physiol, July 2001, Vol. 126, pp. 1174-1185
Altered Expression of SPINDLY Affects Gibberellin
Response and Plant Development1
Stephen M.
Swain,2*
Tong-seung
Tseng, and
Neil E.
Olszewski
Department of Plant Biology and Plant Molecular Genetics Institute,
University of Minnesota, St. Paul, Minnesota 55108
Gibberellins (GAs) are plant hormones with diverse roles in plant
growth and development. SPINDLY (SPY) is
one of several genes identified in Arabidopsis that are involved in GA
response and it is thought to encode an O-GlcNAc
transferase. Genetic analysis suggests that SPY negatively regulates GA
response. To test the hypothesis that SPY acts specifically as a
negatively acting component of GA signal transduction,
spy mutants and plants containing a 35S:SPY construct
have been examined. A detailed investigation of the spy
mutant phenotype suggests that SPY may play a role in plant development
beyond its role in GA signaling. Consistent with this suggestion, the
analysis of spy er plants suggests that the
ERECTA (ER) gene, which has not been
implicated as having a role in GA signaling, appears to enhance the
non-GA spy mutant phenotypes. Arabidopsis plants
containing a 35S:SPY construct possess reduced GA response at seed
germination, but also possess phenotypes consistent with increased GA
response, although not identical to spy mutants, during
later vegetative and reproductive development. Based on these results,
the hypothesis that SPY is specific for GA signaling is rejected.
Instead, it is proposed that SPY is a negative regulator of GA response
that has additional roles in plant development.
1
This work was supported by the National Science
Foundation (grant no. MCB-9604126 to N.E.O.).
2
Present address: Commonwealth Scientific and Industrial
Research Organization Plant Industry, Private Mail Bag, Merbein
3505, Victoria, Australia.
*
Corresponding author; e-mail Steve.Swain{at}pi.csiro.au; fax
61-3-51513111.
© 2001 American Society of Plant Physiologists
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