|
Plant Physiol, July 2001, Vol. 126, pp. 1186-1195
Defoliation Induces Fructan 1-Exohydrolase II in Witloof
Chicory Roots. Cloning and Purification of Two Isoforms, Fructan
1-Exohydrolase IIa and Fructan 1-Exohydrolase IIb. Mass Fingerprint
of the Fructan 1-Exohydrolase II Enzymes1
Wim
Van den Ende,*
An
Michiels,
Dominik
Van Wonterghem,
Stefan P.
Clerens,
Joke
De Roover, and
André J.
Van Laere
Department of Biology, Laboratory for Developmental Biology, Botany
Institute, K.U. Leuven, Kasteelpark Arenberg 31, B-3001 Leuven,
Belgium (W.V.d.E., A.M., D.V.W., J.D.R, A.J.V.L.); Laboratoroy for
Neuroendocrinology and Immunological Biotechnology, Zoological
Institute, K.U. Leuven, Naamsestraat 59, B-3001 Leuven, Belgium
(S.P.C.)
The cloning of two highly homologous chicory (Cichorium
intybus var. foliosum cv Flash) fructan 1-exohydrolase cDNAs
(1-FEH IIa and 1-FEH IIb) is described. Both isoenzymes could be
purified from forced chicory roots as well as from the etiolated
"Belgian endive" leaves where the 1-FEH IIa isoform is present in
higher concentrations. Full-length cDNAs were obtained by a combination of reverse transcriptase-polymerase chain reaction (PCR), PCR and 5'-
and 3'-rapid amplification of cDNA ends using primers based on
N-terminal and conserved amino acid sequences. 1-FEH IIa and 1-FEH IIb
cDNA-derived amino acid sequences are most homologous to a new group of
plant glycosyl hydrolases harboring cell wall-type enzymes with acid
isoelectric points. Unlike the observed expression profiles of chicory
1-FEH I, northern analysis revealed that 1-FEH II is expressed when
young chicory plants are defoliated, suggesting that this enzyme can be
induced at any developmental stage when large energy supplies are
necessary (regrowth after defoliation).
1
This work was supported by the Fund for
Scientific Research, Flanders (grant to W.V.d.E., S.P.C., and
J.D.R.).
*
Corresponding author; e-mail
wim.vandenende{at}bio.kuleuven.ac.be; fax 32-16-321967.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
B. Lasseur, L. Schroeven, W. Lammens, K. Le Roy, G. Spangenberg, H. Manduzio, R. Vergauwen, J. Lothier, M.-P. Prud'homme, and W. Van den Ende
Transforming a Fructan:Fructan 6G-Fructosyltransferase from Perennial Ryegrass into a Sucrose:Sucrose 1-Fructosyltransferase
Plant Physiology,
January 1, 2009;
149(1):
327 - 339.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. F. Asega, J. R. O. do Nascimento, L. Schroeven, W. Van den Ende, and M. A. M. Carvalho
Cloning, Characterization and Functional Analysis of a 1-FEH cDNA from Vernonia herbacea (Vell.) Rusby
Plant Cell Physiol.,
August 1, 2008;
49(8):
1185 - 1195.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Le Roy, W. Lammens, M. Verhaest, B. De Coninck, A. Rabijns, A. Van Laere, and W. Van den Ende
Unraveling the Difference between Invertases and Fructan Exohydrolases: A Single Amino Acid (Asp-239) Substitution Transforms Arabidopsis Cell Wall Invertase1 into a Fructan 1-Exohydrolase
Plant Physiology,
November 1, 2007;
145(3):
616 - 625.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Lothier, B. Lasseur, K. Le Roy, A. Van Laere, M.-P. Prud'homme, P. Barre, W. Van den Ende, and A. Morvan-Bertrand
Cloning, gene mapping, and functional analysis of a fructan 1-exohydrolase (1-FEH) from Lolium perenne implicated in fructan synthesis rather than in fructan mobilization
J. Exp. Bot.,
June 1, 2007;
58(8):
1969 - 1983.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Lasseur, J. Lothier, A. Djoumad, B. De Coninck, S. Smeekens, A. Van Laere, A. Morvan-Bertrand, W. Van den Ende, and M.-P. Prud'homme
Molecular and functional characterization of a cDNA encoding fructan:fructan 6G-fructosyltransferase (6G-FFT)/fructan:fructan 1-fructosyltransferase (1-FFT) from perennial ryegrass (Lolium perenne L.)
J. Exp. Bot.,
August 1, 2006;
57(11):
2719 - 2734.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Van den Ende, S. Clerens, R. Vergauwen, D. Boogaerts, K. Le Roy, L. Arckens, and A. Van Laere
Cloning and functional analysis of a high DP fructan:fructan 1-fructosyl transferase from Echinops ritro (Asteraceae): comparison of the native and recombinant enzymes
J. Exp. Bot.,
March 1, 2006;
57(4):
775 - 789.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Michiels, A. Van Laere, W. Van den Ende, and M. Tucker
Expression analysis of a chicory fructan 1-exohydrolase gene reveals complex regulation by cold
J. Exp. Bot.,
June 1, 2004;
55(401):
1325 - 1333.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Van den Ende, S. Clerens, R. Vergauwen, L. Van Riet, A. Van Laere, M. Yoshida, and A. Kawakami
Fructan 1-Exohydrolases. beta -(2,1)-Trimmers during Graminan Biosynthesis in Stems of Wheat? Purification, Characterization, Mass Mapping, and Cloning of Two Fructan 1-Exohydrolase Isoforms
Plant Physiology,
February 1, 2003;
131(2):
621 - 631.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
