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Plant Physiol, July 2001, Vol. 126, pp. 1299-1313

Class I beta -1,3-Glucanase and Chitinase Are Expressed in the Micropylar Endosperm of Tomato Seeds Prior to Radicle Emergence1

Chun-Ta Wu, Gerhard Leubner-Metzger,2 Frederick Meins Jr., and Kent J. Bradford*

Department of Vegetable Crops, One Shields Avenue, University of California, Davis, California 95616-8631 (C.-T.W., K.J.B.); and Friedrich Miescher-Institut, P.O. Box 2543, CH-4002 Basel, Switzerland (G.L.-M., F.M.)

beta -1,3-Glucanase (EC 3.2.1.39) and chitinase (EC 3.2.1.14) mRNAs, proteins, and enzyme activities were expressed specifically in the micropylar tissues of imbibed tomato (Lycopersicon esculentum Mill.) seeds prior to radicle emergence. RNA hybridization and immunoblotting demonstrated that both enzymes were class I basic isoforms. beta -1,3-Glucanase was expressed exclusively in the endosperm cap tissue, whereas chitinase localized to both endosperm cap and radicle tip tissues. beta -1,3-Glucanase and chitinase appeared in the micropylar tissues of gibberellin-deficient gib-1 tomato seeds only when supplied with gibberellin. Accumulation of beta -1,3-glucanase mRNA, protein and enzyme activity was reduced by 100 µM abscisic acid, which delayed or prevented radicle emergence but not endosperm cap weakening. In contrast, expression of chitinase mRNA, protein, and enzyme activity was not affected by abscisic acid. Neither of these enzymes significantly hydrolyzed isolated tomato endosperm cap cell walls. Although both beta -1,3-glucanase and chitinase were expressed in tomato endosperm cap tissue prior to radicle emergence, we found no evidence that they were directly involved in cell wall modification or tissue weakening. Possible functions of these hydrolases during tomato seed germination are discussed.

1 This research was supported in part by the National Science Foundation (grants IBN-9407264 and IBN-9722978 to K.J.B.).

2 Present address: Institut für Biologie II, Botanik, Schaenzlestrasse 1, D-79104 Freiburg, Germany

* Corresponding author; e-mail kjbradford{at}ucdavis.edu; fax 530-752-4554.

© 2001 American Society of Plant Physiologists


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