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Plant Physiol, July 2001, Vol. 126, pp. 973-980
Cysteine Synthase Overexpression in Tobacco Confers Tolerance to
Sulfur-Containing Environmental Pollutants1
Masaaki
Noji,
Maiko
Saito,
Michimi
Nakamura,
Mitsuko
Aono,
Hikaru
Saji, and
Kazuki
Saito*
Graduate School of Pharmaceutical Sciences, Department of Molecular
Biology and Biotechnology, Chiba University, Yayoi-cho 1-33, Inage-ku,
Chiba 263-8522, Japan (M.N., M.S., M.N., K.S.); and Division of
Environmental Biology, National Institute for Environmental Studies,
16-2 Onogawa, Tsukuba, Ibaraki 305-0053, Japan (M.A.,
H.S.)
Cysteine (Cys) synthase
[O-acetyl-L-Ser(thiol)-lyase, EC 4.2.99.8;
CSase] is responsible for the final step in biosynthesis of Cys.
Transgenic tobacco (Nicotiana tabacum; F1)
plants with enhanced CSase activities in the cytosol and in the
chloroplasts were generated by cross-fertilization of two transformants
expressing cytosolic CSase or chloroplastic CSase. The F1
transgenic plants were highly tolerant to toxic sulfur dioxide and
sulfite. Upon fumigation with 0.1 µL L 1 sulfur dioxide,
the Cys and glutathione contents in leaves of F1 plants
were increased significantly, but not in leaves of non-transformed control plants. Furthermore, the leaves of F1 plants
exhibited the increased resistance to paraquat, a herbicide generating
active oxygen species.
1
This work was supported by grants-in-aid for
scientific research from the Ministry of Education, Science, Sports and
Culture, Japan, by Core Research for Evolutional Science and Technology of Japan Science and Technology, by the Showa Shell Sekiyu Foundation, and by the Asahi Glass Foundation.
*
Corresponding author; e-mail ksaito{at}p.chiba-u.ac.jp; fax
81-43-290-2905.
© 2001 American Society of Plant Physiologists
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