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Plant Physiol, August 2001, Vol. 126, pp. 1370-1380
Overexpression of KNAT1 in Lettuce Shifts Leaf
Determinate Growth to a Shoot-Like Indeterminate Growth Associated with
an Accumulation of Isopentenyl-Type Cytokinins1
Giovanna
Frugis,
Donato
Giannino,
Giovanni
Mele,
Chiara
Nicolodi,
Adriana
Chiappetta,
Maria Beatrice
Bitonti,
Anna Maria
Innocenti,
Walter
Dewitte,
Harry
Van Onckelen, and
Domenico
Mariotti*
Istituto di Biochimica ed Ecofisiologia Vegetali del Consiglio
Nazionale delle Richerche, via Salaria km 29,300-00016 Monterotondo
Scalo, Rome, Italy (G.F., D.G., G.M., C.N., D.M.); Universita' degli
Studi della Calabria, Dipartimento di Ecologia, Laboratorio di
Botanica, Ponte P. Bucci Cubo 6B, 87030 Rende, Cosenza, Italy (A.C.,
M.B.B., A.M.I.); and University of Antwerp, Universiteitsplein 1, B-2610 Antwerpen, Belgium (W.D., H.V.O.)
Leaves are specialized organs characterized by defined
developmental destiny and determinate growth. The overexpression of Knotted1-like homeobox genes in different species has
been shown to alter leaf shape and development, but a definite role for
this class of genes remains to be established. Transgenics that
overexpress Knotted1-like genes present some traits that
are characteristic of altered cytokinin physiology. Here we show that
lettuce (Lactuca sativa) leaves that overexpress
KNAT1, an Arabidopsis kn1-like gene, acquire
characteristics of indeterminate growth typical of the shoot and that
this cell fate change is associated with the accumulation of specific
types of cytokinins. The possibility that the phenotypic effects of
KNAT1 overexpression may arise primarily from the modulation of local
ratios of different cytokinins is discussed.
1
This work was supported by a grant from the
European Community and by the Agriculture, Agro-Industry including
Fisheries project "Lettuce for the Next Century" (contract
no. 92-250). G.F. and D.G. were supported by postdoctoral fellowships
from the Italian National Council of Research.
*
Corresponding author; e-mail mari{at}sun.area.mlib.cnr.it; fax
39-06-9064492.
© 2001 American Society of Plant Physiologists
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