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Plant Physiol, August 2001, Vol. 126, pp. 1438-1448
Hydrogen Peroxide Is Involved in Abscisic Acid-Induced Stomatal
Closure in Vicia faba1
Xiao
Zhang,
Lin
Zhang,
Facai
Dong,
Junfeng
Gao,
David W.
Galbraith, and
Chun-Peng
Song2*
Department of Biology, Henan University, Kaifeng 475001, People's
Republic of China (X.Z., L.Z., F.D., C.-P.S.); Department of
Basic Courses, Northwestern Agricultural University, Yangling 712100, People's Republic of China (X.Z., J.G.); and Department of Plant
Sciences, University of Arizona, Tucson, Arizona 85721 (D.W.G.)
One of the most important functions of the plant hormone abscisic
acid (ABA) is to induce stomatal closure by reducing the turgor of
guard cells under water deficit. Under environmental stresses, hydrogen
peroxide (H2O2), an active oxygen species, is
widely generated in many biological systems. Here, using an epidermal
strip bioassay and laser-scanning confocal microscopy, we provide
evidence that H2O2 may function as an
intermediate in ABA signaling in Vicia faba guard
cells. H2O2 inhibited induced closure
of stomata, and this effect was reversed by ascorbic acid at
concentrations lower than 10 5 M.
Further, ABA-induced stomatal closure also was abolished partly by addition of exogenous catalase (CAT) and diphenylene iodonium (DPI),
which are an H2O2 scavenger and an NADPH
oxidase inhibitor, respectively. Time course experiments of single-cell
assays based on the fluorescent probe dichlorofluorescein showed that
the generation of H2O2 was dependent on ABA
concentration and an increase in the fluorescence intensity of the
chloroplast occurred significantly earlier than within the other
regions of guard cells. The ABA-induced change in fluorescence
intensity in guard cells was abolished by the application of CAT and
DPI. In addition, ABA microinjected into guard cells markedly induced
H2O2 production, which preceded stomatal
closure. These effects were abolished by CAT or DPI micro-injection. Our results suggest that guard cells treated with ABA may close the
stomata via a pathway with H2O2 production
involved, and H2O2 may be an intermediate in
ABA signaling.
1
This work was supported by the National Natural
Science Foundation of China (grant no. 3990407021 to C.-P.S.) and by
the National Key Basic Research Special Funds (grant no. G1999011700 to
C.-P.S.).
2
Present address: Department of Plant Sciences, Forbes
Building 303, University of Arizona, Tucson, AZ 85721.
*
Corresponding author; e-mail songcp{at}ag.arizona.edu; fax
520-621-7186.
© 2001 American Society of Plant Physiologists
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