|
Plant Physiol, August 2001, Vol. 126, pp. 1449-1458
Phospholipase D and Phosphatidic Acid-Mediated Generation of
Superoxide in Arabidopsis1
Yongming
Sang,
Decai
Cui,2 and
Xuemin
Wang*
Department of Biochemistry, Kansas State University, Manhattan,
Kansas 66506
Phospholipase D (PLD), which hydrolyzes phospholipids into free
head groups and phosphatidic acid (PA), may regulate cellular processes
through the production of lipid and lipid-derived messengers. We have
genetically abrogated PLD , the most prevalent isoform of PLD in
plants, and the depletion of PLD in Arabidopsis decreased the levels
of PA and superoxide production in Arabidopsis leaf extracts. Addition
of PA promoted the synthesis of superoxide in the PLD-depleted
plants, as measured by chemiluminescence and superoxide
dismutase-inhibitable, NADPH-dependent reduction of cytochrome c and
nitroblue tetrazolium. The PA-enhanced generation of superoxide was
associated mainly with microsomal membranes. Among various lipids
tested, PA was the most effective stimulator with the optimal
concentrations between 100 and 200 µM. The PA-promoted production of superoxide was observed also in leaves directly infiltrated with PA. The added PA was more effective in stimulating superoxide generation in the PLD-depleted leaves than in the PLD-containing, wild-type leaves, suggesting that PA produced in the
cell was more effective than added PA in promoting superoxide production. These data indicate that PLD plays a role in mediating superoxide production in plants through the generation of PA as a lipid messenger.
1
This work was supported by the National Science
Foundation (grant no. IBN-9808729). This report is contribution no.
01-353-J of the Kansas Agricultural Experiment Station.
2
Present address: Institute of Plant Genetics and
Breeding, Shandong Agricultural University, 61 Daizong Street, Taian,
Shandong 271018, China.
*
Corresponding author; e-mail wangs{at}ksu.edu; fax 785-532-6422.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
Y. Zhang, H. Zhu, Q. Zhang, M. Li, M. Yan, R. Wang, L. Wang, R. Welti, W. Zhang, and X. Wang
Phospholipase D{alpha}1 and Phosphatidic Acid Regulate NADPH Oxidase Activity and Production of Reactive Oxygen Species in ABA-Mediated Stomatal Closure in Arabidopsis
PLANT CELL,
August 1, 2009;
21(8):
2357 - 2377.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yamaguchi, M. Kuroda, H. Yamakawa, T. Ashizawa, K. Hirayae, L. Kurimoto, T. Shinya, and N. Shibuya
Suppression of a Phospholipase D Gene, OsPLD{beta}1, Activates Defense Responses and Increases Disease Resistance in Rice
Plant Physiology,
May 1, 2009;
150(1):
308 - 319.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-Y. Park, J. Jin, Y.-W. Lee, S. Kang, and Y.-H. Lee
Rice Blast Fungus (Magnaporthe oryzae) Infects Arabidopsis via a Mechanism Distinct from That Required for the Infection of Rice
Plant Physiology,
January 1, 2009;
149(1):
474 - 486.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Bustos, R. Lascano, A. L. Villasuso, E. Machado, M. E. Senn, A. Cordoba, and E. Taleisnik
Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2*- Levels
Ann. Bot.,
October 1, 2008;
102(4):
551 - 559.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Hong, S. Zheng, and X. Wang
Dual Functions of Phospholipase D{alpha}1 in Plant Response to Drought
Mol Plant,
March 1, 2008;
1(2):
262 - 269.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Laxalt, N. Raho, A. t. Have, and L. Lamattina
Nitric Oxide Is Critical for Inducing Phosphatidic Acid Accumulation in Xylanase-elicited Tomato Cells
J. Biol. Chem.,
July 20, 2007;
282(29):
21160 - 21168.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. P. Mane, C. Vasquez-Robinet, A. A. Sioson, L. S. Heath, and R. Grene
Early PLD{alpha}-mediated events in response to progressive drought stress in Arabidopsis: a transcriptome analysis
J. Exp. Bot.,
January 1, 2007;
58(2):
241 - 252.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. G. Anthony, S. Khan, J. Costa, M. S. Pais, and L. Bogre
The Arabidopsis Protein Kinase PTI1-2 Is Activated by Convergent Phosphatidic Acid and Oxidative Stress Signaling Pathways Downstream of PDK1 and OXI1
J. Biol. Chem.,
December 8, 2006;
281(49):
37536 - 37546.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Song, I. Steinebrunner, X. Wang, S. C. Stout, and S. J. Roux
Extracellular ATP Induces the Accumulation of Superoxide via NADPH Oxidases in Arabidopsis
Plant Physiology,
April 1, 2006;
140(4):
1222 - 1232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kobayashi, K. Kawakita, M. Maeshima, N. Doke, and H. Yoshioka
Subcellular localization of Strboh proteins and NADPH-dependent O2--generating activity in potato tuber tissues
J. Exp. Bot.,
March 1, 2006;
57(6):
1373 - 1379.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Li, C. Qin, R. Welti, and X. Wang
Double Knockouts of Phospholipases D{zeta}1 and D{zeta}2 in Arabidopsis Affect Root Elongation during Phosphate-Limited Growth But Do Not Affect Root Hair Patterning
Plant Physiology,
February 1, 2006;
140(2):
761 - 770.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Wang
Regulatory Functions of Phospholipase D and Phosphatidic Acid in Plant Growth, Development, and Stress Responses
Plant Physiology,
October 1, 2005;
139(2):
566 - 573.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Williams, J. Torabinejad, E. Cohick, K. Parker, E. J. Drake, J. E. Thompson, M. Hortter, and D. B. DeWald
Mutations in the Arabidopsis Phosphoinositide Phosphatase Gene SAC9 Lead to Overaccumulation of PtdIns(4,5)P2 and Constitutive Expression of the Stress-Response Pathway
Plant Physiology,
June 1, 2005;
138(2):
686 - 700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yamaguchi, E. Minami, J. Ueki, and N. Shibuya
Elicitor-induced Activation of Phospholipases Plays an Important Role for the Induction of Defense Responses in Suspension-cultured Rice Cells
Plant Cell Physiol.,
April 1, 2005;
46(4):
579 - 587.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yamaguchi, S. Tanabe, E. Minami, and N. Shibuya
Activation of Phospholipase D Induced by Hydrogen Peroxide in Suspension-cultured Rice Cells
Plant Cell Physiol.,
September 15, 2004;
45(9):
1261 - 1270.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Zhang, C. Qin, J. Zhao, and X. Wang
Phospholipase D{alpha}1-derived phosphatidic acid interacts with ABI1 phosphatase 2C and regulates abscisic acid signaling
PNAS,
June 22, 2004;
101(25):
9508 - 9513.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zhao and X. Wang
Arabidopsis Phospholipase D{alpha}1 Interacts with the Heterotrimeric G-protein {alpha}-Subunit through a Motif Analogous to the DRY Motif in G-protein-coupled Receptors
J. Biol. Chem.,
January 16, 2004;
279(3):
1794 - 1800.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Park, Y. Gu, Y. Lee, Z. Yang, and Y. Lee
Phosphatidic Acid Induces Leaf Cell Death in Arabidopsis by Activating the Rho-Related Small G Protein GTPase-Mediated Pathway of Reactive Oxygen Species Generation
Plant Physiology,
January 1, 2004;
134(1):
129 - 136.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Wang and X. Wang
A Novel Phospholipase D of Arabidopsis That Is Activated by Oleic Acid and Associated with the Plasma Membrane
Plant Physiology,
November 1, 2001;
127(3):
1102 - 1112.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
