Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Satoh, D.
Right arrow Articles by Matsuda, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Satoh, D.
Right arrow Articles by Matsuda, Y.
Agricola
Right arrow Articles by Satoh, D.
Right arrow Articles by Matsuda, Y.

Plant Physiol, August 2001, Vol. 126, pp. 1459-1470

Physiological and Molecular Biological Characterization of Intracellular Carbonic Anhydrase from the Marine Diatom Phaeodactylum tricornutum1

Dan Satoh,2 Yasutaka Hiraoka,2 Brian Colman,3 and Yusuke Matsuda*

Department of Chemistry, Kwansei-Gakuin University, 1-1-155 Uegahara, Nishinomiya 662-8501, Japan

A single intracellular carbonic anhydrase (CA) was detected in air-grown and, at reduced levels, in high CO2-grown cells of the marine diatom Phaeodactylum tricornutum (UTEX 642). No external CA activity was detected irrespective of growth CO2 conditions. Ethoxyzolamide (0.4 mM), a CA-specific inhibitor, severely inhibited high-affinity photosynthesis at low concentrations of dissolved inorganic carbon, whereas 2 mM acetazolamide had little effect on the affinity for dissolved inorganic carbon, suggesting that internal CA is crucial for the operation of a carbon concentrating mechanism in P. tricornutum. Internal CA was purified 36.7-fold of that of cell homogenates by ammonium sulfate precipitation, and two-step column chromatography on diethylaminoethyl-sephacel and p-aminomethylbenzene sulfone amide agarose. The purified CA was shown, by SDS-PAGE, to comprise an electrophoretically single polypeptide of 28 kD under both reduced and nonreduced conditions. The entire sequence of the cDNA of this CA was obtained by the rapid amplification of cDNA ends method and indicated that the cDNA encodes 282 amino acids. Comparison of this putative precursor sequence with the N-terminal amino acid sequence of the purified CA indicated that it included a possible signal sequence of up to 46 amino acids at the N terminus. The mature CA was found to consist of 236 amino acids and the sequence was homologous to beta -type CAs. Even though the zinc-ligand amino acid residues were shown to be completely conserved, the amino acid residues that may constitute a CO2-binding site appeared to be unique among the beta -CAs so far reported.

1 This work was supported in part by a grant from Invitation for Research Institute of Innovative Technology for the Earth Research Proposals and in part by Kwansei-Gakuin University (Special Grant for Individual Researcher to Y.M. and a visiting professorship to B.C.).

2 These authors contributed equally to the paper.

3 Present address: Department of Biology, York University, 4700 Keele Street, Toronto, Canada M3J 1P3.

* Corresponding author; e-mail yusuke{at}kwansei.ac.jp; fax 81-798-51-0914.

© 2001 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. Lapointe, T. D.B. MacKenzie, and D. Morse
An External {delta}-Carbonic Anhydrase in a Free-Living Marine Dinoflagellate May Circumvent Diffusion-Limited Carbon Acquisition
Plant Physiology, July 1, 2008; 147(3): 1427 - 1436.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Harada, K. Nakajima, K. Sakaue, and Y. Matsuda
CO2 Sensing at Ocean Surface Mediated by cAMP in a Marine Diatom
Plant Physiology, November 1, 2006; 142(3): 1318 - 1328.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
A. R. Soto, H. Zheng, D. Shoemaker, J. Rodriguez, B. A. Read, and T. M. Wahlund
Identification and Preliminary Characterization of Two cDNAs Encoding Unique Carbonic Anhydrases from the Marine Alga Emiliania huxleyi
Appl. Envir. Microbiol., August 1, 2006; 72(8): 5500 - 5511.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
E. G. Mogensen, G. Janbon, J. Chaloupka, C. Steegborn, M. S. Fu, F. Moyrand, T. Klengel, D. S. Pearson, M. A. Geeves, J. Buck, et al.
Cryptococcus neoformans Senses CO2 through the Carbonic Anhydrase Can2 and the Adenylyl Cyclase Cac1
Eukaryot. Cell, January 1, 2006; 5(1): 103 - 111.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Harada, D. Nakatsuma, M. Ishida, and Y. Matsuda
Regulation of the Expression of Intracellular {beta}-Carbonic Anhydrase in Response to CO2 and Light in the Marine Diatom Phaeodactylum tricornutum
Plant Physiology, October 1, 2005; 139(2): 1041 - 1050.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Tanaka, D. Nakatsuma, H. Harada, M. Ishida, and Y. Matsuda
Localization of Soluble {beta}-Carbonic Anhydrase in the Marine Diatom Phaeodactylum tricornutum. Sorting to the Chloroplast and Cluster Formation on the Girdle Lamellae
Plant Physiology, May 1, 2005; 138(1): 207 - 217.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Montsant, K. Jabbari, U. Maheswari, and C. Bowler
Comparative Genomics of the Pennate Diatom Phaeodactylum tricornutum
Plant Physiology, February 1, 2005; 137(2): 500 - 513.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2001 by the American Society of Plant Biologists