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Plant Physiol, August 2001, Vol. 126, pp. 1471-1479
Modification of Expansin Transcript Levels in the Maize Primary
Root at Low Water Potentials1
Yajun
Wu,2
Eleanor T.
Thorne,2
Robert E.
Sharp, and
Daniel J.
Cosgrove*
Department of Biology, 208 Mueller Laboratory, Pennsylvania State
University, University Park, Pennsylvania 16802 (Y.W., D.J.C.); and
Department of Agronomy, Plant Sciences Unit, University of Missouri,
Columbia, Missouri 65211 (E.T.T., R.E.S.)
We previously demonstrated that maintenance of cell elongation in
the apical region of maize primary roots at low water potentials ( w) was associated with an increase in expansin activity
and extractable expansin protein. Here, we characterized the spatial pattern of expansin gene expression along the growing maize root and
studied the effect of low w on expansin gene expression. Roots were divided into three segments: apical 0 to 5 mm, subapical 5 to 10 mm, and non-growing 10 to 20 mm. Of the five expansin genes
expressed in control roots, two  -expansins (Exp1 and
Exp5) and two  -expansins (ExpB2 and
ExpB8) are expressed specifically in the growing region,
whereas expression of -expansin ExpB6 is shifted
basipetally. After seedlings were transplanted to vermiculite with a
w of  1.6 MPa, transcripts for
Exp1, Exp5, and ExpB8
rapidly accumulated in the apical region of the root. These mRNA
changes correlated with the maintenance of root elongation and
increases in wall extensibility found previously. The -expansins
ExpB2 and ExpB6 showed distinctive
patterns of expression and responses to low w,
indicative of distinctive functions. Inhibition of abscisic acid (ABA)
accumulation at low w (by fluridone treatment) had no
effect on expansin expression, except that ExpB2
transcript level showed a minor dependence on ABA. Gene-specific
regulation of - and -expansin mRNA pools likely contributes to
growth alterations of the maize (Zea mays) root as it
adapts to a low w, but these changes do not
appear to be mediated by changes in ABA content.
1
This work was supported by the U.S. Department
of Agriculture National Research Initiative (grant no. PENR-9601307),
by the U.S. Department of Energy (grant no. DE-FG02-84ER13179 to
D.J.C.), and by the University of Missouri Food for the 21st Century
Program (grant to R.E.S.). E.T.T. was supported by a pre-doctoral
fellowship from the U.S. Department of Agriculture National Needs
Training Grant in Plant Biotechnology (no. 98-38420-5834). This is
contribution no. 13,097 from the Missouri Agricultural Experiment
Station journal series.
2
These authors contributed equally to the paper.
*
Corresponding author; e-mail dCosgrove{at}psu.edu; fax
814-865-9131.
© 2001 American Society of Plant Physiologists
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