Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cen, Y.-P.
Right arrow Articles by Layzell, D. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cen, Y.-P.
Right arrow Articles by Layzell, D. B.
Agricola
Right arrow Articles by Cen, Y.-P.
Right arrow Articles by Layzell, D. B.

Plant Physiol, August 2001, Vol. 126, pp. 1555-1565

Whole-Plant Gas Exchange and Reductive Biosynthesis in White Lupin1

Yan-Ping Cen, David H. Turpin,2 and David B. Layzell*

Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6

Simultaneous measurements of CO2 (CER) and O2 (OER) exchange in roots and shoots of vegetative white lupin (Lupinus albus) were used to calculate the flow of reducing power to the synthesis of biomass that was more reduced per unit of carbon than carbohydrate. On a whole-plant basis, the diverted reductant utilization rate (DRUR which is: 4 × [CER + OER]) of shoot tissue was consistently higher than that of roots, and values obtained in the light were greater than those in the dark. An analysis of the biomass being synthesized over a 24-h period provided an estimate of whole-plant DRUR (3.5 mmol e- plant-1 d-1), which was similar to that measured by gas exchange (3.2 mmol e- plant-1 d-1). Given that nitrate reduction to ammonia makes up about 74% of whole-plant DRUR, root nitrate reduction in white lupin was estimated to account for less than 43% of whole-plant nitrate reduction. The approach developed here should offer a powerful tool for the noninvasive study of metabolic regulation in intact plants or plant organs.

1 This work was supported by the Natural Sciences and Engineering Research Council of Canada (research grants to D.B.L. and D.H.T.).

2 Present Address: Department of Biology, University of Victoria, Victoria, BC, Canada V8W 2Y2.

* Corresponding author; e-mail layzelld{at}biology.queensu.ca; fax 613-533-6617.

© 2001 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Rachmilevitch, A. B. Cousins, and A. J. Bloom
Nitrate assimilation in plant shoots depends on photorespiration
PNAS, August 3, 2004; 101(31): 11506 - 11510.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y.-P. Cen and D. B. Layzell
In Vivo Gas Exchange Measurement of the Site and Dynamics of Nitrate Reduction in Soybean
Plant Physiology, March 1, 2003; 131(3): 1147 - 1156.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
I. Scheurwater, M. Koren, H. Lambers, and O. K. Atkin
The contribution of roots and shoots to whole plant nitrate reduction in fast- and slow-growing grass species
J. Exp. Bot., July 1, 2002; 53(374): 1635 - 1642.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2001 by the American Society of Plant Biologists