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Plant Physiol, August 2001, Vol. 126, pp. 1555-1565

Whole-Plant Gas Exchange and Reductive Biosynthesis in White Lupin1

Yan-Ping Cen, David H. Turpin,2 and David B. Layzell*

Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6

Simultaneous measurements of CO2 (CER) and O2 (OER) exchange in roots and shoots of vegetative white lupin (Lupinus albus) were used to calculate the flow of reducing power to the synthesis of biomass that was more reduced per unit of carbon than carbohydrate. On a whole-plant basis, the diverted reductant utilization rate (DRUR which is: 4 × [CER + OER]) of shoot tissue was consistently higher than that of roots, and values obtained in the light were greater than those in the dark. An analysis of the biomass being synthesized over a 24-h period provided an estimate of whole-plant DRUR (3.5 mmol e- plant-1 d-1), which was similar to that measured by gas exchange (3.2 mmol e- plant-1 d-1). Given that nitrate reduction to ammonia makes up about 74% of whole-plant DRUR, root nitrate reduction in white lupin was estimated to account for less than 43% of whole-plant nitrate reduction. The approach developed here should offer a powerful tool for the noninvasive study of metabolic regulation in intact plants or plant organs.

1 This work was supported by the Natural Sciences and Engineering Research Council of Canada (research grants to D.B.L. and D.H.T.).

2 Present Address: Department of Biology, University of Victoria, Victoria, BC, Canada V8W 2Y2.

* Corresponding author; e-mail layzelld{at}biology.queensu.ca; fax 613-533-6617.

© 2001 American Society of Plant Physiologists


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