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Plant Physiol, August 2001, Vol. 126, pp. 1619-1629
The Expression of Alternative Oxidase and Uncoupling Protein
during Fruit Ripening in Mango1
Michael James
Considine,
Daniel Oliver
Daley, and
James
Whelan*
Department of Biochemistry, The University of Western Australia,
Nedlands, Western Australia 6907, Australia
The expression of alternative oxidase (Aox) and uncoupling proteins
(Ucp) was investigated during ripening in mango (Mangifera indica) and compared with the expression of peroxisomal
thiolase, a previously described ripening marker in mango. The
multigene family for the Aox in mango was expressed differentially
during ripening. Abundance of Aox message and protein both peaked at the ripe stage. Expression of the single gene for the Ucp peaked at the
turning stage and the protein abundance peaked at the ripe stage.
Proteins of the cytochrome chain peaked at the mature stage of
ripening. The pattern of protein accumulation suggested that increases
in cytochrome chain components played an important role in facilitating
the climacteric burst of respiration and that the Aox and Ucp may play
a role in post-climacteric senescent processes. Because both message
and protein for the Aox and Ucp increased in a similar pattern, it
suggests that their expression is not controlled in a reciprocal manner
but may be active simultaneously.
1
This research was supported by the Australian
Research Council (grant to J.W.). M.J.C. was sponsored by AgWEST.
*
Corresponding author; e-mail seamus{at}cyllene.uwa.edu.au; fax
61-8-9380-1148.
© 2001 American Society of Plant Physiologists
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