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Plant Physiol, August 2001, Vol. 126, pp. 1637-1645
Expression of 35S::Pto Globally Activates
Defense-Related Genes in Tomato Plants1
Fangming
Xiao,
Xiaoyan
Tang, and
Jian-Min
Zhou*
Department of Plant Pathology, Kansas State University, Manhattan,
Kansas 66506
The tomato (Lycopersicon esculentum) resistance gene
Pto confers resistance to the bacterial pathogen
Pseudomonas syringae pv tomato carrying
the avirulent gene avrPto. Overexpressing
Pto under the control of the cauliflower mosaic virus
35S promoter constitutively activates defense responses in the absence
of pathogen infection and nonspecifically enhances disease resistance.
To elucidate the mechanisms underlying this resistance, we isolated cDNAs corresponding to transcripts that accumulated in
35S::Pto plants. By using suppression
subtractive hybridization, we isolated 82 unique cDNA clones, most of
which corresponded to differentially expressed transcripts. Most of the
genes examined were also induced by pathogen inoculation. Sequence
analysis showed that a large number of genes encode defense-related
proteins, and most had not been previously isolated from tomato. The
isolated cDNAs also include those with a putative role in the oxidative
burst, proteolysis, the hypersensitive response, signal transduction,
and a number of genes with unknown functions. The isolation of these
cDNAs of diverse functions will assist in the characterization of
defense pathways activated during disease resistance.
1
This work was supported by the National Science
Foundation (grant no. MCB9808701 to J.-M.Z.) and by the U.S. Department
of Agriculture (grant no. 9802511 to X.T.). This is Kansas Agricultural Experimental Station contribution no. 01-338-J.
*
Corresponding author; e-mail jzhou{at}plantpath.ksu.edu; fax
785-532-5692.
© 2001 American Society of Plant Physiologists
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