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Plant Physiol, August 2001, Vol. 126, pp. 1668-1677
A Novel Superoxide Dismutase with a High Isoelectric Point in
Higher Plants. Expression, Regulation, and Protein
Localization1
Barbara
Karpinska,2
Marlene
Karlsson,
Helga
Schinkel,
Steffen
Streller,
Karl-Heinz
Süss,3
Michael
Melzer,3 and
Gunnar
Wingsle*
Umeå Plant Science Centre, Department of Forest Genetics
and Plant Physiology, Faculty of Forestry, Swedish University of
Agricultural Sciences, 901 83 Umeå, Sweden
Several isoforms of superoxide dismutase (SOD) with a high
isoelectric point (pI) have been identified by isoelectric focusing chromatography in protein extracts from Scots pine (Pinus
sylvestris) needles. One of these isoforms, a CuZn-SOD with a
pI of about 10 and thus denoted hipI-SOD, has been isolated and
purified to apparent homogeneity. A cDNA encoding the hipI-SOD
protein was cloned and sequenced. Northern hybridization of mRNA
isolated from different organs and tissues showed that hipI-SOD has a
markedly different pattern of expression compared with chloroplastic
and cytosolic SOD. Furthermore, the transcript levels of hipI-SOD and
cytosolic SOD were found to respond differently to mechanical wounding,
treatment with oxidized glutathione, paraquat, and ozone. Immunogold
electron microscopy localized the hipI-SOD in the plasma membrane of
sieve cells and the Golgi apparatus of albuminous cells. Moreover, high
protein density was also detected in extracellular spaces such as
secondary cell wall thickenings of the xylem and sclerenchyma and in
intercellular spaces of parenchyma cells.
1
This work was supported by the Swedish Councils
for Forestry and Agricultural Research, Natural Sciences, and Strategic
Research (grants to Swedish University of Agricultured Sciences).
2
Present address: Department of Botany, Stockholm
University, Lilla Frescativägen 5, Frescati, SE-106 91 Stockholm, Sweden.
3
Present address: Department of Molecular Cell Biology,
Institute of Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany.
*
Corresponding author; e-mail Gunnar.Wingsle{at}genfys.slu.se; fax
46-90-786-59-01.
© 2001 American Society of Plant Physiologists
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