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Plant Physiol, September 2001, Vol. 127, pp. 14-22
BIN2, a New Brassinosteroid-Insensitive Locus in
Arabidopsis1
Jianming
Li,*
Kyoung Hee
Nam,
Dionne
Vafeados, and
Joanne
Chory
Department of Biology, University of Michigan, Ann Arbor, Michigan
48109-1048 (J.L., K.H.N.); and The Howard Hughes Medical Institute and
The Plant Biology Laboratory, The Salk Institute for Biological
Studies, La Jolla, California 92037 (D.V., J.C.)
Brassinosteroids (BRs) play important roles throughout plant
development. Although many genes have been identified that are involved
in BR biosynthesis, genetic approaches in Arabidopsis have led to the
identification of only one gene, BRI1, that encodes a
membrane receptor for BRs. To expand our knowledge of the molecular mechanism(s) of plant steroid signaling, we analyzed many dwarf and
semidwarf mutants collected from our previous genetic screens and
identified a semidwarf mutant that showed little response to exogenous
BR treatments. Genetic analysis of the bin2
(BR-INSENSITIVE 2) mutant indicated that the
BR-insensitive dwarf phenotype was due to a semidominant mutation in
the BIN2 gene that mapped to the middle of chromosome IV
between the markers CH42 and AG. A direct screening for similar
semidwarf mutants resulted in the identification of a second allele of
the BIN2 gene. Despite some novel phenotypes observed
with the bin2/+ mutants, the homozygous bin2 mutants were almost identical to the
well-characterized bri1 mutants that are defective in BR
perception. In addition to the BR-insensitive dwarf phenotype,
bin2 mutants exhibited BR insensitivity when assayed for
root growth inhibition and feedback inhibition of CPD
gene expression. Furthermore, bin2 mutants displayed an abscisic acid-hypersensitive phenotype that is shared by the
bri1 and BR-deficient mutants. A gene dosage experiment
using triploid plants suggested that the bin2 phenotypes
were likely caused by either neomorphic or hypermorphic
gain-of-function mutations in the BIN2 gene. Thus, the
two bin2 mutations define a novel genetic locus whose
gene product might play a role in BR signaling.
1
This work was supported in part by a University
of Michigan start-up fund (to J.L.), by a University of Michigan
Rackham Faculty Award (to J.L.), by the National Institutes of Health
(grant no. GM60519 to J.L.), and by the U.S. Department of Agriculture
(grant no. 99-35301-7903 to J.C.). J.C. is an Associate Investigator of the Howard Hughes Medical Institute.
*
Corresponding author; e-mail jian{at}umich.edu; fax
734-647-0884.
© 2001 American Society of Plant Physiologists
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|
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
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162(3):
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[Full Text]
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|
|
|
|
|
|
|
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|
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|
|
|
|
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|
|
|
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|
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297(5582):
793 - 797.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-X. He, J. M. Gendron, Y. Yang, J. Li, and Z.-Y. Wang
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10185 - 10190.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yin, H. Cheong, D. Friedrichsen, Y. Zhao, J. Hu, S. Mora-Garcia, and J. Chory
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PNAS,
July 23, 2002;
99(15):
10191 - 10196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. J. Bishop and C. Koncz
Brassinosteroids and Plant Steroid Hormone Signaling
PLANT CELL,
May 1, 2002;
14(90001):
S97 - 110.
[Full Text]
[PDF]
|
|
|
|
|
