Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (48)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ganeteg, U.
Right arrow Articles by Jansson, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ganeteg, U.
Right arrow Articles by Jansson, S.
Agricola
Right arrow Articles by Ganeteg, U.
Right arrow Articles by Jansson, S.

Plant Physiol, September 2001, Vol. 127, pp. 150-158

The Properties of the Chlorophyll a/b-Binding Proteins Lhca2 and Lhca3 Studied in Vivo Using Antisense Inhibition1

Ulrika Ganeteg,* Åsa Strand, Petter Gustafsson, and Stefan Jansson

Umeå Plant Science Center, Department of Plant Physiology, Umeå University, S-901 87 Umeå, Sweden

The specific functions of the light-harvesting proteins Lhca2 and Lhca3 were studied in Arabidopsis ecotype Colombia antisense plants in which the proteins were individually repressed. The antisense effect was specific in each plant, but levels of Lhca proteins other than the targeted products were also affected. The contents of Lhca1 and Lhca4 were unaffected, but Lhca3 (in Lhca2-repressed plants) was almost completely depleted, and Lhca2 decreased to about 30% of wild-type levels in Lhca3-repressed plants. This suggests that the Lhca2 and Lhca3 proteins are in physical contact with each other and that they require each other for stability. Photosystem I fluorescence at 730 nm is thought to emanate from pigments bound to Lhca1 and Lhca4. However, fluorescence emission and excitation spectra suggest that Lhca2 and Lhca3, which fluoresce in vitro at 680 nm, also could contribute to far-red fluorescence in vivo. Spectral forms with absorption maxima at 695 and 715 nm, apparently with emission maxima at 702 and 735 nm, respectively, might be associated with Lhca2 and Lhca3.

1 This work was supported by grants from the Swedish Forestry and Agricultural Research Council.

* Corresponding author; e-mail ulrika.ganeteg{at}plantphys.umu.se; fax 46-90-7866676.

© 2001 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
E. Wientjes, G. T. Oostergetel, S. Jansson, E. J. Boekema, and R. Croce
The Role of Lhca Complexes in the Supramolecular Organization of Higher Plant Photosystem I
J. Biol. Chem., March 20, 2009; 284(12): 7803 - 7810.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Kovacs, J. Damkjaer, S. Kereiche, C. Ilioaia, A. V. Ruban, E. J. Boekema, S. Jansson, and P. Horton
Lack of the Light-Harvesting Complex CP24 Affects the Structure and Function of the Grana Membranes of Higher Plant Chloroplasts
PLANT CELL, November 1, 2006; 18(11): 3106 - 3120.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. L.E. Sjogren, T. M. Stanne, B. Zheng, S. Sutinen, and A. K. Clarke
Structural and Functional Insights into the Chloroplast ATP-Dependent Clp Protease in Arabidopsis
PLANT CELL, October 1, 2006; 18(10): 2635 - 2649.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. V. Ruban, S. Solovieva, P. J. Lee, C. Ilioaia, M. Wentworth, U. Ganeteg, F. Klimmek, W. S. Chow, J. M. Anderson, S. Jansson, et al.
Plasticity in the Composition of the Light Harvesting Antenna of Higher Plants Preserves Structural Integrity and Biological Function
J. Biol. Chem., May 26, 2006; 281(21): 14981 - 14990.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Morosinotto, M. Ballottari, F. Klimmek, S. Jansson, and R. Bassi
The Association of the Antenna System to Photosystem I in Higher Plants: COOPERATIVE INTERACTIONS STABILIZE THE SUPRAMOLECULAR COMPLEX AND ENHANCE RED-SHIFTED SPECTRAL FORMS
J. Biol. Chem., September 2, 2005; 280(35): 31050 - 31058.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Naumann, E. J. Stauber, A. Busch, F. Sommer, and M. Hippler
N-terminal Processing of Lhca3 Is a Key Step in Remodeling of the Photosystem I-Light-harvesting Complex Under Iron Deficiency in Chlamydomonas reinhardtii
J. Biol. Chem., May 27, 2005; 280(21): 20431 - 20441.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Storf, S. Jansson, and V. H. R. Schmid
Pigment Binding, Fluorescence Properties, and Oligomerization Behavior of Lhca5, a Novel Light-harvesting Protein
J. Biol. Chem., February 18, 2005; 280(7): 5163 - 5168.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. E. Jensen, A. Haldrup, S. Zhang, and H. V. Scheller
The PSI-O Subunit of Plant Photosystem I Is Involved in Balancing the Excitation Pressure between the Two Photosystems
J. Biol. Chem., June 4, 2004; 279(23): 24212 - 24217.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
U. Ganeteg, C. Kulheim, J. Andersson, and S. Jansson
Is Each Light-Harvesting Complex Protein Important for Plant Fitness?
Plant Physiology, January 1, 2004; 134(1): 502 - 509.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. E. Desquilbet, J.-C. Duval, B. Robert, J. Houmard, and J. C. Thomas
In the Unicellular Red Alga Rhodella violacea Iron Deficiency Induces an Accumulation of Uncoupled LHC
Plant Cell Physiol., November 15, 2003; 44(11): 1141 - 1151.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. N. Melkozernov and R. E. Blankenship
Structural Modeling of the Lhca4 Subunit of LHCI-730 Peripheral Antenna in Photosystem I Based on Similarity with LHCII
J. Biol. Chem., November 7, 2003; 278(45): 44542 - 44551.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Wissel, F. Pettersson, A. Berglund, and S. Jansson
What Affects mRNA Levels in Leaves of Field-Grown Aspen? A Study of Developmental and Environmental Influences
Plant Physiology, November 1, 2003; 133(3): 1190 - 1197.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Zolla, S. Rinalducci, A. M. Timperio, and C. G. Huber
Proteomics of Light-Harvesting Proteins in Different Plant Species. Analysis and Comparison by Liquid Chromatography-Electrospray Ionization Mass Spectrometry. Photosystem I
Plant Physiology, December 1, 2002; 130(4): 1938 - 1950.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. H. R. Schmid, S. Potthast, M. Wiener, V. Bergauer, H. Paulsen, and S. Storf
Pigment Binding of Photosystem I Light-harvesting Proteins
J. Biol. Chem., September 27, 2002; 277(40): 37307 - 37314.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Varotto, P. Pesaresi, P. Jahns, A. Lessnick, M. Tizzano, F. Schiavon, F. Salamini, and D. Leister
Single and Double Knockouts of the Genes for Photosystem I Subunits G, K, and H of Arabidopsis. Effects on Photosystem I Composition, Photosynthetic Electron Flow, and State Transitions
Plant Physiology, June 1, 2002; 129(2): 616 - 624.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. E. Jensen, L. Rosgaard, J. Knoetzel, and H. Vibe Scheller
Photosystem I Activity Is Increased in the Absence of the PSI-G Subunit
J. Biol. Chem., January 18, 2002; 277(4): 2798 - 2803.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2001 by the American Society of Plant Biologists