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Plant Physiol, September 2001, Vol. 127, pp. 23-32
Specific Association of Transcripts of tbzF and
tbz17, Tobacco Genes Encoding Basic Region Leucine
Zipper-Type Transcriptional Activators, with Guard Cells of
Senescing Leaves and/or Flowers1
Seung Hwan
Yang,
Thomas
Berberich,
Hiroshi
Sano, and
Tomonobu
Kusano2*
Research and Education Center for Genetic Information, Nara
Institute of Science and Technology, Ikoma, Nara 630-0101, Japan
(S.H.Y., H.S., T.K.); and Botanisches Institut,
Goethe-Universität, Postfach 11 19 32, D-60054 Frankfurt am
Main, Germany (T.B.)
Induction by low temperature is a common feature of the
lip19 subfamily members of the basic region leucine
zipper gene family in plants. Here, we characterize two tobacco
(Nicotiana tabacum) genes, tbzF and
tbz17, belonging to the lip19 subfamily,
whose gene products, TBZF and TBZ17, show 73% identity and are located in nuclei. They preferentially bind to DNA fragments spanning A-box/G-box and C-box/G-box hybrid motifs and show transactivation activity in cobombarded tobacco BY-2 cells, indicating they function as
transcriptional activators. Transcripts of tbzF were
detected at a high level in senescing leaves and flowers. In contrast, tbz17 transcripts could be shown to accumulate in aged
leaves but not in flowers. In situ hybridization analysis revealed
transcripts of tbzF and tbz17 to be
predominantly located in guard cells and vascular tissues of senescing
leaves. These results suggest that TBZF and TBZ17 are both involved in
controlling gene transcription related to functions of guard cells in
senescing leaves and that TBZF bifunctionally acts in floral development.
1
This work was supported in part by the Japan
Society for Promotion of Science (grant nos. JSPS-RFTF96L00602 and
JSPS-RFTF 00L01604) and by the Deutsche Forschungsgemeinschaft (grant
no. Be 2183/2-1).
2
Present address: Graduate School of Life Sciences,
Tohoku University, 2-1-1 Katahira, Aoba, Sendai, Miyagi 980-8577, Japan.
*
Corresponding author; e-mail kusano{at}ige.tohoku.ac.jp; fax
81-22-217-5709.
© 2001 American Society of Plant Physiologists
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