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Plant Physiol, September 2001, Vol. 127, pp. 262-271

Major Alterations of the Regulation of Root NO3minus Uptake Are Associated with the Mutation of Nrt2.1 and Nrt2.2 Genes in Arabidopsis1

Miguel Cerezo,2 Pascal Tillard, Sophie Filleur, Stéphane Muños, Françoise Daniel-Vedele, and Alain Gojon*

Biochimie et Physiologie Moléculaire des Plantes, Unité Mixte de Recherche 5004, Agro-Montpellier/Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Université Montpellier 2, Place Viala, 34060 Montpellier cedex, France (M.C., P.T., S.M., A.G.); and Nutrition Azotée des Plantes, Institut National de la Recherche Agronomique, Route de St-Cyr, 78026 Versailles cedex, France (S.F., F.D-.V.)

The role of AtNrt2.1 and AtNrt2.2 genes, encoding putative NO3- transporters in Arabidopsis, in the regulation of high-affinity NO3- uptake has been investigated in the atnrt2 mutant, where these two genes are deleted. Our initial analysis of the atnrt2 mutant (S. Filleur, M.F. Dorbe, M. Cerezo, M. Orsel, F. Granier, A. Gojon, F. Daniel-Vedele [2001] FEBS Lett 489: 220-224) demonstrated that root NO3- uptake is affected in this mutant due to the alteration of the high-affinity transport system (HATS), but not of the low-affinity transport system. In the present work, we show that the residual HATS activity in atnrt2 plants is not inducible by NO3-, indicating that the mutant is more specifically impaired in the inducible component of the HATS. Thus, high-affinity NO3- uptake in this genotype is likely to be due to the constitutive HATS. Root 15NO3- influx in the atnrt2 mutant is no more derepressed by nitrogen starvation or decrease in the external NO3- availability. Moreover, the mutant also lacks the usual compensatory up-regulation of NO3- uptake in NO3--fed roots, in response to nitrogen deprivation of another portion of the root system. Finally, exogenous supply of NH4+ in the nutrient solution fails to inhibit 15NO3- influx in the mutant, whereas it strongly decreases that in the wild type. This is not explained by a reduced activity of NH4+ uptake systems in the mutant. These results collectively indicate that AtNrt2.1 and/or AtNrt2.2 genes play a key role in the regulation of the high-affinity NO3- uptake, and in the adaptative responses of the plant to both spatial and temporal changes in nitrogen availability in the environment.

1 This work was supported by the Spanish Ministerio de Educación y Cultura, Subprograma de Perfeccionamiento de Doctores y Tecnólogos en el extranjero (Boletín Official del Estado 25/09/99).

2 Present address: Unidad de Biotecnologia Vegetal, Departamento de Ciencias Experimentales, Escuela Superior de Tecnologia y Ciencias Experimentales, Universitat Jaume I, 12071 Castellon, Spain.

* Corresponding author; e-mail gojon{at}ensam.inra.fr; fax 33-4-67-52-57-37.

© 2001 American Society of Plant Physiologists


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