Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (44)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cerezo, M.
Right arrow Articles by Gojon, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cerezo, M.
Right arrow Articles by Gojon, A.
Agricola
Right arrow Articles by Cerezo, M.
Right arrow Articles by Gojon, A.

Plant Physiol, September 2001, Vol. 127, pp. 262-271

Major Alterations of the Regulation of Root NO3minus Uptake Are Associated with the Mutation of Nrt2.1 and Nrt2.2 Genes in Arabidopsis1

Miguel Cerezo,2 Pascal Tillard, Sophie Filleur, Stéphane Muños, Françoise Daniel-Vedele, and Alain Gojon*

Biochimie et Physiologie Moléculaire des Plantes, Unité Mixte de Recherche 5004, Agro-Montpellier/Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/Université Montpellier 2, Place Viala, 34060 Montpellier cedex, France (M.C., P.T., S.M., A.G.); and Nutrition Azotée des Plantes, Institut National de la Recherche Agronomique, Route de St-Cyr, 78026 Versailles cedex, France (S.F., F.D-.V.)

The role of AtNrt2.1 and AtNrt2.2 genes, encoding putative NO3- transporters in Arabidopsis, in the regulation of high-affinity NO3- uptake has been investigated in the atnrt2 mutant, where these two genes are deleted. Our initial analysis of the atnrt2 mutant (S. Filleur, M.F. Dorbe, M. Cerezo, M. Orsel, F. Granier, A. Gojon, F. Daniel-Vedele [2001] FEBS Lett 489: 220-224) demonstrated that root NO3- uptake is affected in this mutant due to the alteration of the high-affinity transport system (HATS), but not of the low-affinity transport system. In the present work, we show that the residual HATS activity in atnrt2 plants is not inducible by NO3-, indicating that the mutant is more specifically impaired in the inducible component of the HATS. Thus, high-affinity NO3- uptake in this genotype is likely to be due to the constitutive HATS. Root 15NO3- influx in the atnrt2 mutant is no more derepressed by nitrogen starvation or decrease in the external NO3- availability. Moreover, the mutant also lacks the usual compensatory up-regulation of NO3- uptake in NO3--fed roots, in response to nitrogen deprivation of another portion of the root system. Finally, exogenous supply of NH4+ in the nutrient solution fails to inhibit 15NO3- influx in the mutant, whereas it strongly decreases that in the wild type. This is not explained by a reduced activity of NH4+ uptake systems in the mutant. These results collectively indicate that AtNrt2.1 and/or AtNrt2.2 genes play a key role in the regulation of the high-affinity NO3- uptake, and in the adaptative responses of the plant to both spatial and temporal changes in nitrogen availability in the environment.

1 This work was supported by the Spanish Ministerio de Educación y Cultura, Subprograma de Perfeccionamiento de Doctores y Tecnólogos en el extranjero (Boletín Official del Estado 25/09/99).

2 Present address: Unidad de Biotecnologia Vegetal, Departamento de Ciencias Experimentales, Escuela Superior de Tecnologia y Ciencias Experimentales, Universitat Jaume I, 12071 Castellon, Spain.

* Corresponding author; e-mail gojon{at}ensam.inra.fr; fax 33-4-67-52-57-37.

© 2001 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 Plant CellHome page
A. Almagro, S. H. Lin, and Y. F. Tsay
Characterization of the Arabidopsis Nitrate Transporter NRT1.6 Reveals a Role of Nitrate in Early Embryo Development
PLANT CELL, December 1, 2008; 20(12): 3289 - 3299.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Kant, Y.-M. Bi, E. Weretilnyk, S. Barak, and S. J. Rothstein
The Arabidopsis Halophytic Relative Thellungiella halophila Tolerates Nitrogen-Limiting Conditions by Maintaining Growth, Nitrogen Uptake, and Assimilation
Plant Physiology, July 1, 2008; 147(3): 1168 - 1180.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Ruffel, S. Freixes, S. Balzergue, P. Tillard, C. Jeudy, M. L. Martin-Magniette, M. J. van der Merwe, K. Kakar, J. Gouzy, A. R. Fernie, et al.
Systemic Signaling of the Plant Nitrogen Status Triggers Specific Transcriptome Responses Depending on the Nitrogen Source in Medicago truncatula
Plant Physiology, April 1, 2008; 146(4): 2020 - 2035.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Lejay, J. Wirth, M. Pervent, J. M.-F. Cross, P. Tillard, and A. Gojon
Oxidative Pentose Phosphate Pathway-Dependent Sugar Sensing as a Mechanism for Regulation of Root Ion Transporters by Photosynthesis
Plant Physiology, April 1, 2008; 146(4): 2036 - 2053.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Richard-Molard, A. Krapp, F. Brun, B. Ney, F. Daniel-Vedele, and S. Chaillou
Plant response to nitrate starvation is determined by N storage capacity matched by nitrate uptake capacity in two Arabidopsis genotypes
J. Exp. Bot., March 1, 2008; 59(4): 779 - 791.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Wirth, F. Chopin, V. Santoni, G. Viennois, P. Tillard, A. Krapp, L. Lejay, F. Daniel-Vedele, and A. Gojon
Regulation of Root Nitrate Uptake at the NRT2.1 Protein Level in Arabidopsis thaliana
J. Biol. Chem., August 10, 2007; 282(32): 23541 - 23552.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. Camanes, M. Cerezo, E. Primo-Millo, A. Gojon, and P. Garcia-Agustin
Ammonium transport and CitAMT1 expression are regulated by light and sucrose in Citrus plants
J. Exp. Bot., August 1, 2007; 58(11): 2811 - 2825.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Yuan, D. Loque, S. Kojima, S. Rauch, K. Ishiyama, E. Inoue, H. Takahashi, and N. von Wiren
The Organization of High-Affinity Ammonium Uptake in Arabidopsis Roots Depends on the Spatial Arrangement and Biochemical Properties of AMT1-Type Transporters
PLANT CELL, August 1, 2007; 19(8): 2636 - 2652.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F. Chopin, M. Orsel, M.-F. Dorbe, F. Chardon, H.-N. Truong, A. J. Miller, A. Krapp, and F. Daniel-Vedele
The Arabidopsis ATNRT2.7 Nitrate Transporter Controls Nitrate Content in Seeds
PLANT CELL, May 1, 2007; 19(5): 1590 - 1602.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Remans, P. Nacry, M. Pervent, S. Filleur, E. Diatloff, E. Mounier, P. Tillard, B. G. Forde, and A. Gojon
The Arabidopsis NRT1.1 transporter participates in the signaling pathway triggering root colonization of nitrate-rich patches
PNAS, December 12, 2006; 103(50): 19206 - 19211.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Krouk, P. Tillard, and A. Gojon
Regulation of the High-Affinity NO3- Uptake System by NRT1.1-Mediated NO3- Demand Signaling in Arabidopsis
Plant Physiology, November 1, 2006; 142(3): 1075 - 1086.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Orsel, F. Chopin, O. Leleu, S. J. Smith, A. Krapp, F. Daniel-Vedele, and A. J. Miller
Characterization of a Two-Component High-Affinity Nitrate Uptake System in Arabidopsis. Physiology and Protein-Protein Interaction
Plant Physiology, November 1, 2006; 142(3): 1304 - 1317.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Remans, P. Nacry, M. Pervent, T. Girin, P. Tillard, M. Lepetit, and A. Gojon
A Central Role for the Nitrate Transporter NRT2.1 in the Integrated Morphological and Physiological Responses of the Root System to Nitrogen Limitation in Arabidopsis
Plant Physiology, March 1, 2006; 140(3): 909 - 921.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Okamoto, A. Kumar, W. Li, Y. Wang, M. Y. Siddiqi, N. M. Crawford, and A. D.M. Glass
High-Affinity Nitrate Transport in Roots of Arabidopsis Depends on Expression of the NAR2-Like Gene AtNRT3.1
Plant Physiology, March 1, 2006; 140(3): 1036 - 1046.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Munos, C. Cazettes, C. Fizames, F. Gaymard, P. Tillard, M. Lepetit, L. Lejay, and A. Gojon
Transcript Profiling in the chl1-5 Mutant of Arabidopsis Reveals a Role of the Nitrate Transporter NRT1.1 in the Regulation of Another Nitrate Transporter, NRT2.1
PLANT CELL, September 1, 2004; 16(9): 2433 - 2447.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Fizames, S. Munos, C. Cazettes, P. Nacry, J. Boucherez, F. Gaymard, D. Piquemal, V. Delorme, T. Commes, P. Doumas, et al.
The Arabidopsis Root Transcriptome by Serial Analysis of Gene Expression. Gene Identification Using the Genome Sequence
Plant Physiology, January 1, 2004; 134(1): 67 - 80.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Malagoli, P. Laine, E. Le Deunff, L. Rossato, B. Ney, and A. Ourry
Modeling Nitrogen Uptake in Oilseed Rape cv Capitol during a Growth Cycle Using Influx Kinetics of Root Nitrate Transport Systems and Field Experimental Data
Plant Physiology, January 1, 2004; 134(1): 388 - 400.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Lejay, X. Gansel, M. Cerezo, P. Tillard, C. Muller, A. Krapp, N. von Wiren, F. Daniel-Vedele, and A. Gojon
Regulation of Root Ion Transporters by Photosynthesis: Functional Importance and Relation with Hexokinase
PLANT CELL, September 1, 2003; 15(9): 2218 - 2232.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Santi, G. Locci, R. Monte, R. Pinton, and Z. Varanini
Induction of nitrate uptake in maize roots: expression of a putative high-affinity nitrate transporter and plasma membrane H+-ATPase isoforms
J. Exp. Bot., August 1, 2003; 54(389): 1851 - 1864.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Loque, P. Tillard, A. Gojon, and M. Lepetit
Gene Expression of the NO3- Transporter NRT1.1 and the Nitrate Reductase NIA1 Is Repressed in Arabidopsis Roots by NO2-, the Product of NO3- Reduction
Plant Physiology, June 1, 2003; 132(2): 958 - 967.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Okamoto, J. J. Vidmar, and A. D. M. Glass
Regulation of NRT1 and NRT2 Gene Families of Arabidopsis thaliana: Responses to Nitrate Provision
Plant Cell Physiol., March 15, 2003; 44(3): 304 - 317.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Orsel, A. Krapp, and F. Daniel-Vedele
Analysis of the NRT2 Nitrate Transporter Family in Arabidopsis. Structure and Gene Expression
Plant Physiology, June 1, 2002; 129(2): 886 - 896.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Orsel, S. Filleur, V. Fraisier, and F. Daniel-Vedele
Nitrate transport in plants: which gene and which control?
J. Exp. Bot., April 15, 2002; 53(370): 825 - 833.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F.-Q. Guo, R. Wang, and N. M. Crawford
The Arabidopsis dual-affinity nitrate transporter gene AtNRT1.1 (CHL1) is regulated by auxin in both shoots and roots
J. Exp. Bot., April 15, 2002; 53(370): 835 - 844.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2001 by the American Society of Plant Biologists