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Plant Physiol, September 2001, Vol. 127, pp. 345-359
Nitrate-Induced Genes in Tomato Roots. Array Analysis Reveals
Novel Genes That May Play a Role in Nitrogen
Nutrition1,[w]
Yi-Hong
Wang,
David F.
Garvin, and
Leon V.
Kochian*
United States Plant, Soil, and Nutrition Laboratory, United States
Department of Agriculture-Agricultural Research Service, Cornell
University, Ithaca, New York 14853
A subtractive tomato (Lycopersicon esculentum)
root cDNA library enriched in genes up-regulated by changes in plant
mineral status was screened with labeled mRNA from roots of both
nitrate-induced and mineral nutrient-deficient ( nitrogen [N],
phosphorus, potassium [K], sulfur, magnesium, calcium,
iron, zinc, and copper) tomato plants. A subset of cDNAs was
selected from this library based on mineral nutrient-related changes in
expression. Additional cDNAs were selected from a second
mineral-deficient tomato root library based on sequence homology to
known genes. These selection processes yielded a set of 1,280 mineral
nutrition-related cDNAs that were arrayed on nylon membranes for
further analysis. These high-density arrays were hybridized with mRNA
from tomato plants exposed to nitrate at different time points after N
was withheld for 48 h, for plants that were grown on
nitrate/ammonium for 5 weeks prior to the withholding of N. One
hundred-fifteen genes were found to be up-regulated by nitrate
resupply. Among these genes were several previously identified as
nitrate responsive, including nitrate transporters, nitrate and nitrite
reductase, and metabolic enzymes such as transaldolase, transketolase,
malate dehydrogenase, asparagine synthetase, and histidine
decarboxylase. We also identified 14 novel nitrate-inducible genes,
including: (a) water channels, (b) root phosphate and K+
transporters, (c) genes potentially involved in transcriptional regulation, (d) stress response genes, and (e) ribosomal protein genes.
In addition, both families of nitrate transporters were also found to
be inducible by phosphate, K, and iron deficiencies. The identification
of these novel nitrate-inducible genes is providing avenues of research
that will yield new insights into the molecular basis of plant N
nutrition, as well as possible networking between the regulation of N,
phosphorus, and K nutrition.
1
This work was supported by the Agricultural
Research Service Agricultural Genome Program (funding to L.V.K. and
D.F.G.).
[w]
The online version of this article contains Web-only
data. The supplemental material is available at www.plantphysiol.org.
*
Corresponding author; e-mail Lvk1{at}cornell.edu, fax
607-255-2459.
© 2001 American Society of Plant Physiologists
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