Plant Physiol, September 2001, Vol. 127, pp. 58-66

Analysis of the Ethylene Response in the epinastic Mutant of Tomato¹

Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, New York 14853 (C.S.B., E.A.F., J.J.G.); Department of Horticultural Sciences, Texas A&M University, College Station, Texas 77843-2133 (H.-c.Y., S.L., J.J.G.); Plant Science Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, United Kingdom (T.-j.Y., D.G.); and United States Department of Agriculture-Agricultural Research Service Plant, Soil, and Nutrition Laboratory, Tower Road, Ithaca, New York 14853 (J.J.G.)

Ethylene can alter plant morphology due to its effect on cell expansion. The most widely documented example of ethylene-mediated cell expansion is promotion of the "triple response" of seedlings grown in the dark in ethylene. Roots and hypocotyls become shorter and thickened compared with controls due to a reorientation of cell expansion, and curvature of the apical hook is more pronounced. The epinastic (epi) mutant of tomato (Lycopersicon esculentum) has a dark-grown seedling phenotype similar to the triple response even in the absence of ethylene. In addition, in adult plants both the leaves and the petioles display epinastic curvature and there is constitutive expression of an ethylene-inducible chitinase gene. However, petal senescence and abscission and fruit ripening are all normal in epi. A double mutant (epi/epi;Nr/Nr) homozygous for both the recessive epi and dominant ethylene-insensitive Never-ripe loci has the same dark-grown seedling and vegetative phenotypes as epi but possesses the senescence and ripening characteristics of Never-ripe. These data suggest that a subset of ethylene responses controlling vegetative growth and development may be constitutively activated in epi. In addition, the epi locus has been placed on the tomato RFLP map on the long arm of chromosome 4 and does not demonstrate linkage to reported tomato CTR1 homologs.