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Plant Physiol, October 2001, Vol. 127, pp. 505-516

Effects of PSAG12-IPT Gene Expression on Development and Senescence in Transgenic Lettuce1

Matthew S. McCabe,2 Lee C. Garratt,2 Frank Schepers, Wilco J.R.M. Jordi, Geert M. Stoopen, Evert Davelaar, J. Hans A. van Rhijn, J. Brian Power, and Michael R. Davey*

Plant Science Division, School of Biosciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (M.S.M., L.C.G., J.B.P., M.R.D.); Advanta Research, P.O. Box 54-2690 AB, The Netherlands (F.S.); Plant Research International, Post Box 14, 6700 AA Wageningen, The Netherlands (W.J.R.M.J., G.M.S., E.D.); and State Institute for Quality Control of Agricultural Products (RIKILT-DLO), Post Box 230, 6700 AE Wageningen, The Netherlands (J.H.A.v.R.)

An ipt gene under control of the senescence-specific SAG12 promoter from Arabidopsis (PSAG12-IPT) significantly delayed developmental and postharvest leaf senescence in mature heads of transgenic lettuce (Lactuca sativa L. cv Evola) homozygous for the transgene. Apart from retardation of leaf senescence, mature, 60-d-old plants exhibited normal morphology with no significant differences in head diameter or fresh weight of leaves and roots. Induction of senescence by nitrogen starvation rapidly reduced total nitrogen, nitrate, and growth of transgenic and azygous (control) plants, but chlorophyll was retained in the lower (outer) leaves of transgenic plants. Harvested PSAG12-IPT heads also retained chlorophyll in their lower leaves. During later development (bolting and preflowering) of transgenic plants, the decrease in chlorophyll, total protein, and Rubisco content in leaves was abolished, resulting in a uniform distribution of these components throughout the plants. Homozygous PSAG12-IPT lettuce plants showed a slight delay in bolting (4-6 d), a severe delay in flowering (4-8 weeks), and premature senescence of their upper leaves. These changes correlated with significantly elevated concentrations of cytokinin and hexoses in the upper leaves of transgenic plants during later stages of development, implicating a relationship between cytokinin and hexose concentrations in senescence.

1 This work was supported by the EC (grant no. FAIR CT 97-3161). This work was performed under license nos. PHL 18/2462(10/19970), 18/2806(9/1998), and 18A/3155(8/1999) issued by the Ministry of Agriculture, Fisheries, and Food, UK.

2 These authors contributed equally to the paper.

* Corresponding author; e-mail mike.davey{at}nottingham.ac.uk; fax 44-115-9513298.

© 2001 American Society of Plant Physiologists


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