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Plant Physiol, October 2001, Vol. 127, pp. 655-664
Multiple, Distinct Isoforms of Sucrose Synthase in
Pea1
D.H. Paul
Barratt,
Lorraine
Barber,
Nicholas J.
Kruger,
Alison M.
Smith,*
Trevor L.
Wang, and
Cathie
Martin
John Innes Centre, Colney Lane, Norwich NR4 7UH, United Kingdom
(D.H.P.B., L.B., A.M.S., T.L.W., C.M.); and Department of Plant
Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB,
United Kingdom (N.J.K.)
Genes encoding three isoforms of sucrose synthase (Sus1, Sus2, and
Sus3) have been cloned from pea (Pisum sativum). The
genes have distinct patterns of expression in different organs of the plant, and during organ development. Studies of the isoforms expressed as recombinant proteins in Escherichia coli show that
they differ in kinetic properties. Although not of great magnitude, the
differences in properties are consistent with some differentiation of
physiological function between the isoforms. Evidence for
differentiation of function in vivo comes from the phenotypes of
rug4 mutants of pea, which carry mutations in the gene
encoding Sus1. One mutant line (rug4-c) lacks detectable
Sus1 protein in both the soluble and membrane-associated fractions of
the embryo, and Sus activity in the embryo is reduced by 95%. The
starch content of the embryo is reduced by 30%, but the cellulose
content is unaffected. The results imply that different isoforms of Sus
may channel carbon from sucrose towards different metabolic fates
within the cell.
1
This work was supported by a competitive grant
from the Resource Allocation and Stress in Plants Initiative of the
Biotechnology and Biological Sciences Research Council (United
Kingdom), and by the Biotechnology and Biological Sciences Research
Council (Competitive Strategic Grant to the John Innes Centre).
*
Corresponding author; e-mail alison.smith{at}bbrsc.ac.uk; fax
44-0-1603-450045.
© 2001 American Society of Plant Physiologists
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