Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (49)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pittman, J. K.
Right arrow Articles by Hirschi, K. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pittman, J. K.
Right arrow Articles by Hirschi, K. D.
Agricola
Right arrow Articles by Pittman, J. K.
Right arrow Articles by Hirschi, K. D.

Plant Physiol, November 2001, Vol. 127, pp. 1020-1029

Regulation of CAX1, an Arabidopsis Ca2+/H+ Antiporter. Identification of an N-Terminal Autoinhibitory Domain1

Jon K. Pittman and Kendal D. Hirschi*

Plant Physiology Group, U.S. Department of Agriculture/Agricultural Research Service Children's Nutrition Research Center, Baylor College of Medicine, 1100 Bates Street, Houston, Texas 77030 (J.K.P., K.D.H.); and Vegetable and Fruit Improvement Center, Texas A&M University, College Station, Texas 77845 (K.D.H.)

Regulation of Ca2+ transport determines the duration of a Ca2+ signal, and hence, the nature of the biological response. Ca2+/H+ antiporters such as CAX1 (cation exchanger 1), play a key role in determining cytosolic Ca2+ levels. Analysis of a full-length CAX1 clone suggested that the CAX1 open reading frame contains an additional 36 amino acids at the N terminus that were not found in the original clone identified by suppression of yeast (Saccharomyces cerevisiae) vacuolar Ca2+ transport mutants. The long CAX1 (lCAX1) could not suppress the yeast Ca2+ transport defects despite localization to the yeast vacuole. Calmodulin could not stimulate lCAX1 Ca2+/H+ transport in yeast; however, minor alterations in the 36-amino acid region restored Ca2+/H+ transport. Sequence analysis suggests that a 36-amino acid N-terminal regulatory domain may be present in all Arabidopsis CAX-like genes. Together, these results suggest a structural feature involved in regulation of Ca2+/H+ antiport.

1 This work was supported by the U.S. Department of Agriculture/Agricultural Research Service (under Cooperative Agreement No. 58-6250-6001) and by the National Institutes of Health (grant nos. CHRC 5 P30 and 1R01 GM57427).

* Corresponding author; e-mail kendalh{at}bcm.tmc.edu; fax 713-798-7078.

© 2001 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. Zhao, T. Shigaki, H. Mei, Y.-q. Guo, N.-H. Cheng, and K. D. Hirschi
Interaction between Arabidopsis Ca2+/H+ Exchangers CAX1 and CAX3
J. Biol. Chem., February 13, 2009; 284(7): 4605 - 4615.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. K. Pittman, C. Edmond, P. A. Sunderland, and C. M. Bray
A Cation-regulated and Proton Gradient-dependent Cation Transporter from Chlamydomonas reinhardtii Has a Role in Calcium and Sodium Homeostasis
J. Biol. Chem., January 2, 2009; 284(1): 525 - 533.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Morris, H. Tian, S. Park, C. S. Sreevidya, J. M. Ward, and K. D. Hirschi
AtCCX3 Is an Arabidopsis Endomembrane H+-Dependent K+ Transporter
Plant Physiology, November 1, 2008; 148(3): 1474 - 1486.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Morris, K. M. Hawthorne, T. Hotze, S. A. Abrams, and K. D. Hirschi
Nutritional impact of elevated calcium transport activity in carrots
PNAS, February 5, 2008; 105(5): 1431 - 1435.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
H. Mei, J. Zhao, J. K. Pittman, J. Lachmansingh, S. Park, and K. D. Hirschi
In planta regulation of the Arabidopsis Ca2+/H+ antiporter CAX1
J. Exp. Bot., September 26, 2007; (2007) erm190v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Kamiya, T. Akahori, M. Ashikari, and M. Maeshima
Expression of the Vacuolar Ca2+/H+ Exchanger, OsCAX1a, in Rice: Cell and Age Specificity of Expression, and Enhancement by Ca2+
Plant Cell Physiol., January 1, 2006; 47(1): 96 - 106.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Park, N. H. Cheng, J. K. Pittman, K. S. Yoo, J. Park, R. H. Smith, and K. D. Hirschi
Increased Calcium Levels and Prolonged Shelf Life in Tomatoes Expressing Arabidopsis H+/Ca2+ Transporters
Plant Physiology, November 1, 2005; 139(3): 1194 - 1206.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Kamiya, T. Akahori, and M. Maeshima
Expression Profile of the Genes for Rice Cation/H+ Exchanger Family and Functional Analysis in Yeast
Plant Cell Physiol., October 1, 2005; 46(10): 1735 - 1740.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Shigaki, B. J. Barkla, M. C. Miranda-Vergara, J. Zhao, O. Pantoja, and K. D. Hirschi
Identification of a Crucial Histidine Involved in Metal Transport Activity in the Arabidopsis Cation/H+ Exchanger CAX1
J. Biol. Chem., August 26, 2005; 280(34): 30136 - 30142.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N.-H. Cheng, J. K. Pittman, T. Shigaki, J. Lachmansingh, S. LeClere, B. Lahner, D. E. Salt, and K. D. Hirschi
Functional Association of Arabidopsis CAX1 and CAX3 Is Required for Normal Growth and Ion Homeostasis
Plant Physiology, August 1, 2005; 138(4): 2048 - 2060.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G. Schaaf, A. Schikora, J. Haberle, G. Vert, U. Ludewig, J.-F. Briat, C. Curie, and N. von Wiren
A Putative Function for the Arabidopsis Fe-Phytosiderophore Transporter Homolog AtYSL2 in Fe and Zn Homeostasis
Plant Cell Physiol., May 1, 2005; 46(5): 762 - 774.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Waditee, G. S. Hossain, Y. Tanaka, T. Nakamura, M. Shikata, J. Takano, T. Takabe, and T. Takabe
Isolation and Functional Characterization of Ca2+/H+ Antiporters from Cyanobacteria
J. Biol. Chem., February 6, 2004; 279(6): 4330 - 4338.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N.-H. Cheng, J. K. Pittman, J.-K. Zhu, and K. D. Hirschi
The Protein Kinase SOS2 Activates the Arabidopsis H+/Ca2+ Antiporter CAX1 to Integrate Calcium Transport and Salt Tolerance
J. Biol. Chem., January 23, 2004; 279(4): 2922 - 2926.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Kamiya and M. Maeshima
Residues in Internal Repeats of the Rice Cation/H+ Exchanger Are Involved in the Transport and Selection of Cations
J. Biol. Chem., January 2, 2004; 279(1): 812 - 819.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Yamaguchi, M. P. Apse, H. Shi, and E. Blumwald
Topological analysis of a plant vacuolar Na+/H+ antiporter reveals a luminal C terminus that regulates antiporter cation selectivity
PNAS, October 14, 2003; 100(21): 12510 - 12515.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N.-H. Cheng and K. D. Hirschi
Cloning and Characterization of CXIP1, a Novel PICOT Domain-containing Arabidopsis Protein That Associates with CAX1
J. Biol. Chem., February 14, 2003; 278(8): 6503 - 6509.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Shigaki, J. K. Pittman, and K. D. Hirschi
Manganese Specificity Determinants in the Arabidopsis Metal/H+ Antiporter CAX2
J. Biol. Chem., February 14, 2003; 278(8): 6610 - 6617.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N.-H. Cheng, J. K. Pittman, B. J. Barkla, T. Shigaki, and K. D. Hirschi
The Arabidopsis cax1 Mutant Exhibits Impaired Ion Homeostasis, Development, and Hormonal Responses and Reveals Interplay among Vacuolar Transporters
PLANT CELL, February 1, 2003; 15(2): 347 - 364.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. K. Pittman, C. S. Sreevidya, T. Shigaki, H. Ueoka-Nakanishi, and K. D. Hirschi
Distinct N-Terminal Regulatory Domains of Ca2+/H+ Antiporters
Plant Physiology, October 1, 2002; 130(2): 1054 - 1062.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
W. K. Versaw and M. J. Harrison
A Chloroplast Phosphate Transporter, PHT2;1, Influences Allocation of Phosphate within the Plant and Phosphate-Starvation Responses
PLANT CELL, August 1, 2002; 14(8): 1751 - 1766.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. K. Pittman, T. Shigaki, N.-H. Cheng, and K. D. Hirschi
Mechanism of N-terminal Autoinhibition in the Arabidopsis Ca2+/H+ Antiporter CAX1
J. Biol. Chem., July 12, 2002; 277(29): 26452 - 26459.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. Sanders, J. Pelloux, C. Brownlee, and J. F. Harper
Calcium at the Crossroads of Signaling
PLANT CELL, May 1, 2002; 14(90001): S401 - 417.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
N.-h. Cheng, J. K. Pittman, T. Shigaki, and K. D. Hirschi
Characterization of CAX4, an Arabidopsis H+/Cation Antiporter
Plant Physiology, April 1, 2002; 128(4): 1245 - 1254.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2001 by the American Society of Plant Biologists