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Plant Physiol, November 2001, Vol. 127, pp. 1030-1043
Response of Arabidopsis to Iron Deficiency Stress as Revealed by
Microarray Analysis1
Oliver
Thimm,
Bernd
Essigmann,
Sebastian
Kloska,
Thomas
Altmann, and
Thomas J.
Buckhout*
Applied Botany, Humboldt University Berlin, Invalidenstrasse 42, 10115 Berlin, Germany (O.T., T.J.B.); and Max Planck Institute of
Molecular Plant Physiology, Am Mühlenberg 1, 14476 Golm,
Germany (B.E., S.K., T.A.)
Gene expression in response to Fe deficiency was analyzed in
Arabidopsis roots and shoots through the use of a cDNA collection representing at least 6,000 individual gene sequences. Arabidopsis seedlings were grown 1, 3, and 7 d in the absence of Fe, and gene expression in roots and shoots was investigated. Following confirmation of data and normalization methods, expression of several sequences encoding enzymes known to be affected by Fe deficiency was investigated by microarray analysis. Confirmation of literature reports,
particularly for changes in enzyme activity, was not always possible,
but changes in gene expression could be confirmed. An expression
analysis of genes in glycolysis, the tricarboxylic acid cycle,
and oxidative pentose phosphate pathway revealed an induction of
several enzymes within 3 d of Fe-deficient growth, indicating an
increase in respiration in response to Fe deficiency. In roots,
transcription of sequences corresponding to enzymes of anaerobic
respiration was also induced, whereas in shoots, the induction of
several genes in gluconeogenesis, starch degradation, and phloem
loading was observed. Thus, it seemed likely that the energy demand in
roots required for the Fe deficiency response exceeded the capacity of
oxidative phosphorylation, and an increase in carbon import and
anaerobic respiration were required to maintain metabolism.
1
This work was supported in part by the Deutsche
Forschungsgemeinschaft (to T.A. and T.J.B.).
*
Corresponding author; e-mail h1131dqy{at}rz.hu-berlin.de; fax
49-03-2093-8725.
© 2001 American Society of Plant Physiologists
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