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Plant Physiol, November 2001, Vol. 127, pp. 1167-1179
Inhibition of Plastocyanin to P700+
Electron Transfer in Chlamydomonas reinhardtii by
Hyperosmotic Stress1
Jeffrey A.
Cruz,
Brian A.
Salbilla,2
Atsuko
Kanazawa, and
David M.
Kramer*
Institute of Biological Chemistry and Department of Biochemistry
and Biophysics, 289 Clark Hall, Washington State University, Pullman,
Washington 99164-6340
Oxygen electrode and fluorescence studies demonstrate that
linear electron transport in the freshwater alga Chlamydomonas reinhardtii can be completely abolished by abrupt hyperosmotic shock. We show that the most likely primary site of inhibition of
electron transfer by hyperosmotic shock is a blockage of electron transfer between plastocyanin (PC) or cytochrome
c6 and P700. The effects
on this reaction were reversible upon dilution of the osmolytes and the
stability of plastocyanin or photosystem (PS) I was unaffected.
Electron micrographs of osmotically shocked cells showed a significant
decrease in the thylakoid lumen volume. Comparison of estimated lumenal
width with the x-ray structures of plastocyanin and PS I suggest that
lumenal space contracts during HOS so as to hinder the movement
of docking to PS I of plastocyanin or cytochrome
c6.
1
This work was supported by the U.S.
Department of Agriculture National Research Initiative Competitive
Grants Program (grant no. 9635306577), by the Plant
Biochemistry Research Training Center (postdoctoral fellowship no.
DE-FG06-94ER20160 to J.A.C.), and by the U.S. Department of Energy
(grant no. DE-FG03-98ERZ0299).
2
Present address: Department of Biology, California State
University, Northridge, CA 91330.
*
Corresponding author; e-mail dkramer{at}wsu.edu; fax
509-335-7643.
© 2001 American Society of Plant Physiologists
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