|
Plant Physiol, November 2001, Vol. 127, pp. 1256-1265
Enhanced Levels of the Aroma and Flavor Compound
S-Linalool by Metabolic Engineering of the Terpenoid
Pathway in Tomato Fruits1
Efraim
Lewinsohn,*
Fernond
Schalechet,
Jack
Wilkinson,2
Kenji
Matsui,3
Yaakov
Tadmor,
Kyoung-Hee
Nam,
Orit
Amar,
Elena
Lastochkin,
Olga
Larkov,
Uzi
Ravid,
William
Hiatt,
Shimon
Gepstein, and
Eran
Pichersky
Newe Ya'ar Research Center, Agricultural Research
Organization, Ramat Yishay 30095, Israel (E.L., F.S., Y.T.,
O.A., E.L., O.L., U.R.); Department of Biology, The Technion, Israel
Institute of Technology, Israel (F.S., O.A., S.G.); Monsanto/Calgene
Site, 1920 Fifth Street, Davis, California 95616 (J.W., K.M., W.H.);
and Department of Molecular, Cellular and Developmental Biology,
University of Michigan, Ann Arbor, Michigan (K.-H.N., E.P.)
The aromas of fruits, vegetables, and flowers are mixtures
of volatile metabolites, often present in parts per billion levels or
less. We show here that tomato (Lycopersicon esculentum
Mill.) plants transgenic for a heterologous Clarkia breweri
S-linalool synthase (LIS) gene, under the
control of the tomato late-ripening-specific E8
promoter, synthesize and accumulate S-linalool and
8-hydroxylinalool in ripening fruits. Apart from the difference in
volatiles, no other phenotypic alterations were noted, including the
levels of other terpenoids such as  - and  -tocopherols, lycopene,
 -carotene, and lutein. Our studies indicate that it is possible to
enhance the levels of monoterpenes in ripening fruits by metabolic engineering.
1
This work was supported in part by the U.S.
Israel Binational Agricultural Research and Development fund (grant no.
IS-2709-96). This is publication no. 105/2001 of the Agricultural
Research Organization, Bet Dagan Israel.
2
Present address: Maxygen, Inc., 515 Galveston Drive,
Redwood City, CA 94063.
3
Present address: Department of Biological Chemistry,
Yamaguchi University, Yamaguchi 753-8515, Japan.
*
Corresponding author; e-mail twefraim{at}netvision.net.il; fax
972-4-983-6936.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
M. Saladie, A. J. Matas, T. Isaacson, M. A. Jenks, S. M. Goodwin, K. J. Niklas, R. Xiaolin, J. M. Labavitch, K. A. Shackel, A. R. Fernie, et al.
A Reevaluation of the Key Factors That Influence Tomato Fruit Softening and Integrity
Plant Physiology,
June 1, 2007;
144(2):
1012 - 1028.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Loivamaki, F. Gilmer, R. J. Fischbach, C. Sorgel, A. Bachl, A. Walter, and J.-P. Schnitzler
Arabidopsis, a Model to Study Biological Functions of Isoprene Emission?
Plant Physiology,
June 1, 2007;
144(2):
1066 - 1078.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Hernandez, D. Molenaar, J. Beekwilder, H. Bouwmeester, and J. E. T. van Hylckama Vlieg
Expression of Plant Flavor Genes in Lactococcus lactis
Appl. Envir. Microbiol.,
March 1, 2007;
73(5):
1544 - 1552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Carrari and A. R. Fernie
Metabolic regulation underlying tomato fruit development
J. Exp. Bot.,
June 1, 2006;
57(9):
1883 - 1897.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Beekwilder, M. Alvarez-Huerta, E. Neef, F. W.A. Verstappen, H. J. Bouwmeester, and A. Aharoni
Functional Characterization of Enzymes Forming Volatile Esters from Strawberry and Banana
Plant Physiology,
August 1, 2004;
135(4):
1865 - 1878.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Li, Y. Zhao, B. C. McCaig, B. A. Wingerd, J. Wang, M. E. Whalon, E. Pichersky, and G. A. Howe
The Tomato Homolog of CORONATINE-INSENSITIVE1 Is Required for the Maternal Control of Seed Maturation, Jasmonate-Signaled Defense Responses, and Glandular Trichome Development
PLANT CELL,
January 1, 2004;
16(1):
126 - 143.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Lucker, W. Schwab, B. van Hautum, J. Blaas, L. H. W. van der Plas, H. J. Bouwmeester, and H. A. Verhoeven
Increased and Altered Fragrance of Tobacco Plants after Metabolic Engineering Using Three Monoterpene Synthases from Lemon
Plant Physiology,
January 1, 2004;
134(1):
510 - 519.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ohara, T. Ujihara, T. Endo, F. Sato, and K. Yazaki
Limonene production in tobacco with Perilla limonene synthase cDNA
J. Exp. Bot.,
December 1, 2003;
54(393):
2635 - 2642.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Aharoni, A. P. Giri, S. Deuerlein, F. Griepink, W.-J. de Kogel, F. W. A. Verstappen, H. A. Verhoeven, M. A. Jongsma, W. Schwab, and H. J. Bouwmeester
Terpenoid Metabolism in Wild-Type and Transgenic Arabidopsis Plants
PLANT CELL,
December 1, 2003;
15(12):
2866 - 2884.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Shalit, I. Guterman, H. Volpin, E. Bar, T. Tamari, N. Menda, Z. Adam, D. Zamir, A. Vainstein, D. Weiss, et al.
Volatile Ester Formation in Roses. Identification of an Acetyl-Coenzyme A. Geraniol/Citronellol Acetyltransferase in Developing Rose Petals
Plant Physiology,
April 1, 2003;
131(4):
1868 - 1876.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Lavid, J. Wang, M. Shalit, I. Guterman, E. Bar, T. Beuerle, N. Menda, S. Shafir, D. Zamir, Z. Adam, et al.
O-Methyltransferases Involved in the Biosynthesis of Volatile Phenolic Derivatives in Rose Petals
Plant Physiology,
August 1, 2002;
129(4):
1899 - 1907.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Vainstein, E. Lewinsohn, E. Pichersky, and D. Weiss
Floral Fragrance. New Inroads into an Old Commodity
Plant Physiology,
December 1, 2001;
127(4):
1383 - 1389.
[Full Text]
[PDF]
|
|
|
|
|
