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Plant Physiol, November 2001, Vol. 127, pp. 1299-1309
Isolation and Characterization of a New Peroxiredoxin from Poplar
Sieve Tubes That Uses Either Glutaredoxin or Thioredoxin as a Proton
Donor1
Nicolas
Rouhier,
Eric
Gelhaye,
Pierre-Eric
Sautiere,
Annick
Brun,
Pascal
Laurent,
Denis
Tagu,
Joelle
Gerard,
Elisabeth
de Fa ,
Yves
Meyer, and
Jean-Pierre
Jacquot*
Unité Mixte de Recherche Interaction Arbres
Microorganisms, Institut National de la Recherche
Agronomique-Université Henri Poincaré Nancy I. Biochimie et Biologie Moléculaire Végétales,
Université Henri Poincaré, 54506 Vandoeuvre cedex, France
(N.R., E.G., A.B., P.L., D.T., J.G., E.d.F., J.-P.J.); Laboratoire
d'Endocrinologie des Annélides Equipe Enseignement
Supérieur Associé 97, Université des Sciences
et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France
(P.-E.S.); and Laboratoire de Physiologie et de Biologie
Moléculaire des Plantes, Unité Mixte de Recherche 5545, Université de Perpignan, 66025 Perpignan cedex, France
(Y.M.)
A sequence coding for a peroxiredoxin (Prx) was isolated
from a xylem/phloem cDNA library from Populus trichocarpa
and subsequently inserted into an expression plasmid yielding the
construction pET-Prx. The recombinant protein was produced in
Escherichia coli cells and purified to homogeneity with
a high yield. The poplar Prx is composed of 162 residues, a property
that makes it the shortest plant Prx sequence isolated so far. It was
shown that the protein is monomeric and possesses two conserved
cysteines (Cys). The Prx degrades hydrogen peroxide and alkyl
hydroperoxides in the presence of an exogenous proton donor that can be
either thioredoxin or glutaredoxin (Grx). Based on this finding, we
propose that the poplar protein represents a new type of Prx that
differs from the so-called 2-Cys and 1-Cys Prx, a suggestion supported by the existence of natural fusion sequences constituted of a Prx motif
coupled to a Grx motif. The protein was shown to be highly expressed in
sieve tubes where thioredoxin h and Grx are also major
proteins.
1
This work was supported by Centre National de la
Recherche Scientifique grant no. PCV 98-099 and by the credit
d'installation from the Ministère de l'Education Nationale, de
la Recherche et de la Technologie.
*
Corresponding author; e-mail j2p{at}scbiol.uhp-nancy.fr; fax 33383912243.
© 2001 American Society of Plant Physiologists
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