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Plant Physiol, November 2001, Vol. 127, pp. 749-764
High-Resolution Metabolic Phenotyping of Genetically and
Environmentally Diverse Potato Tuber Systems. Identification of
Phenocopies
Ute
Roessner,
Lothar
Willmitzer, and
Alisdair R.
Fernie*
Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am
Mühlenberg 1, 14476 Golm, Germany
We conducted a comprehensive metabolic phenotyping of potato
(Solanum tuberosum L. cv Desiree) tuber tissue that had
been modified either by transgenesis or exposure to different
environmental conditions using a recently developed gas
chromatography-mass spectrometry profiling protocol. Applying this
technique, we were able to identify and quantify the major constituent
metabolites of the potato tuber within a single chromatographic run.
The plant systems that we selected to profile were tuber discs
incubated in varying concentrations of fructose, sucrose, and mannitol
and transgenic plants impaired in their starch biosynthesis. The
resultant profiles were then compared, first at the level of individual metabolites and then using the statistical tools hierarchical cluster
analysis and principal component analysis. These tools allowed us to
assign clusters to the individual plant systems and to determine
relative distances between these clusters; furthermore, analyzing the
loadings of these analyses enabled identification of the most important
metabolites in the definition of these clusters. The metabolic profiles
of the sugar-fed discs were dramatically different from the wild-type
steady-state values. When these profiles were compared with one another
and also with those we assessed in previous studies, however, we were
able to evaluate potential phenocopies. These comparisons highlight the
importance of such an approach in the functional and qualitative
assessment of diverse systems to gain insights into important mediators
of metabolism.
*
Corresponding author; e-mail Fernie{at}mpimp-golm.mpg.de; fax
49-331-5678408.
© 2001 American Society of Plant Physiologists
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