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Plant Physiol, November 2001, Vol. 127, pp. 910-917
Components of the Arabidopsis C-Repeat/Dehydration-Responsive
Element Binding Factor Cold-Response Pathway Are Conserved in
Brassica napus and Other Plant
Species1
Kirsten R.
Jaglo,2
Susanne
Kleff,3
Keenan L.
Amundsen,
Xin
Zhang,4
Volker
Haake,
James Z.
Zhang,
Thomas
Deits, and
Michael F.
Thomashow*
Department of Crop and Soil Science, Michigan State University,
East Lansing, Michigan 48824 (K.R.J., K.L.A., X.Z., M.F.T.); MBI
International, Lansing, Michigan 48909 (S.K., T.D.); Mendel
Biotechnology Inc., Hayward, California 94545 (V.H., J.Z.Z.);
and Michigan State University-Department of Energy Plant Research
Laboratory (M.F.T), Michigan State University, East Lansing, Michigan
48824
Many plants increase in freezing tolerance in response to low,
nonfreezing temperatures, a phenomenon known as cold acclimation. Cold
acclimation in Arabidopsis involves rapid cold-induced expression of
the C-repeat/dehydration-responsive element binding factor (CBF)
transcriptional activators followed by expression of CBF-targeted genes
that increase freezing tolerance. Here, we present evidence for a CBF
cold-response pathway in Brassica napus. We show that B. napus encodes CBF-like genes and that
transcripts for these genes accumulate rapidly in response to low
temperature followed closely by expression of the cold-regulated
Bn115 gene, an ortholog of the Arabidopsis CBF-targeted
COR15a gene. Moreover, we show that constitutive
overexpression of the Arabidopsis CBF genes in
transgenic B. napus plants induces expression of
orthologs of Arabidopsis CBF-targeted genes and increases the freezing
tolerance of both nonacclimated and cold-acclimated plants. Transcripts encoding CBF-like proteins were also found to accumulate rapidly in
response to low temperature in wheat (Triticum aestivum
L. cv Norstar) and rye (Secale cereale L. cv Puma),
which cold acclimate, as well as in tomato (Lycopersicon
esculentum var. Bonny Best, Castle Mart, Micro-Tom, and D
Huang), a freezing-sensitive plant that does not cold acclimate. An
alignment of the CBF proteins from Arabidopsis, B.
napus, wheat, rye, and tomato revealed the presence of
conserved amino acid sequences, PKK/RPAGRxKFxETRHP and DSAWR,
that bracket the AP2/EREBP DNA binding domains of the proteins and
distinguish them from other members of the AP2/EREBP protein family. We
conclude that components of the CBF cold-response pathway are highly
conserved in flowering plants and not limited to those that cold acclimate.
1
This research was supported by a subcontract
(no. 593-0219-06) under the U.S. Department of
Agriculture/Cooperative State Research, Education, and Extension
Service Cooperative Agreement North Central Biotechnology
Initiative (no. 96-34340-2711), by Mendel Biotechnology, Inc., and by
the Michigan Agricultural Experiment Station.
2
Present address: Campus Box 0448, Department of
Biochemistry and Biophysics, University of California, San Francisco,
CA 94143-0448.
3
Present address: 341 Food Safety Building, Michigan
State University, East Lansing, MI 48824.
4
Home institution: Horticultural Research Institute,
Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin 150086, China.
*
Corresponding author; e-mail thomash6{at}msu.edu; fax
517-353-9168.
© 2001 American Society of Plant Physiologists
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