|
Plant Physiol, November 2001, Vol. 127, pp. 928-936
Two Tomato Expansin Genes Show Divergent Expression and
Localization in Embryos during Seed Development and
Germination1
Feng
Chen,2
Peetambar
Dahal, and
Kent J.
Bradford*
Department of Vegetable Crops, One Shields Avenue, University of
California, Davis, California 95616-8631
Expansins are plant proteins that can induce extension of isolated
cell walls and are proposed to mediate cell expansion. Three expansin
genes were expressed in germinating tomato (Lycopersicon esculentum Mill.) seeds, one of which (LeEXP4)
was expressed specifically in the endosperm cap tissue enclosing the
radicle tip. The other two genes (LeEXP8 and
LeEXP10) were expressed in the embryo and are further
characterized here. LeEXP8 mRNA was not detected in developing or mature seeds but accumulated specifically in the radicle
cortex during and after germination. In contrast,
LeEXP10 mRNA was abundant at an early stage of seed
development corresponding to the period of rapid embryo expansion; it
then decreased during seed maturation and increased again during
germination. When gibberellin-deficient (gib-1) mutant
seeds were imbibed in water, LeEXP8 mRNA was not detected, but a low level of LeEXP10 mRNA was present.
Expression of both genes increased when gib-1 seeds were
imbibed in gibberellin. Abscisic acid did not prevent the initial
expression of LeEXP8 and LeEXP10, but
mRNA abundance of both genes subsequently decreased during extended
incubation. The initial increase in LeEXP8, but not
LeEXP10, mRNA accumulation was blocked by low water
potential, but LeEXP10 mRNA amounts fell after longer
incubation. When seeds were transferred from abscisic acid or low water
potential solutions to water, abundance of both LeEXP8
and LeEXP10 mRNAs increased in association with
germination. The tissue localization and expression patterns of both
LeEXP8 and LeEXP10 suggest
developmentally specific roles during embryo and seedling growth.
1
This work was supported by the National Science
Foundation (grant no. IBN-9722978) and by the U.S. Department of
Agriculture-National Research Initiative Competitive Grants Program
(grant no. 2000-01434 to K.J.B.).
2
Present address: Department of Biology, University of
Michigan, 830 North University, Kraus Natural Science Building, Ann Arbor, MI 48109-1048.
*
Corresponding author; e-mail kjbradford{at}ucdavis.edu; fax
530-752-4554.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
G. W. Bassel, R. T. Mullen, and J. D. Bewley
procera is a putative DELLA mutant in tomato (Solanum lycopersicum): effects on the seed and vegetative plant
J. Exp. Bot.,
February 3, 2008;
(2008)
erm354v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. V. Arana, M. J. Burgin, L. C. de Miguel, and R. A. Sanchez
The very-low-fluence and high-irradiance responses of the phytochromes have antagonistic effects on germination, mannan-degrading activities, and DfGA3ox transcript levels in Datura ferox seeds
J. Exp. Bot.,
November 15, 2007;
(2007)
erm256v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. V. A. Pinto, E. A. A. Da silva, A. C. Davide, V. A. M. De Jesus, P. E. Toorop, and H. W. M. Hilhorst
Mechanism and Control of Solanum lycocarpum Seed Germination
Ann. Bot.,
November 1, 2007;
100(6):
1175 - 1187.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. T. Ayele, J. A. Ozga, L. V. Kurepin, and D. M. Reinecke
Developmental and Embryo Axis Regulation of Gibberellin Biosynthesis during Germination and Young Seedling Growth of Pea
Plant Physiology,
November 1, 2006;
142(3):
1267 - 1281.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. H. Yennawar, L.-C. Li, D. M. Dudzinski, A. Tabuchi, and D. J. Cosgrove
Inaugural Article: Crystal structure and activities of EXPB1 (Zea m 1), a beta-expansin and group-1 pollen allergen from maize
PNAS,
October 3, 2006;
103(40):
14664 - 14671.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. A. da Silva, P. E. Toorop, J. Nijsse, J. D. Bewley, and H. W. M. Hilhorst
Exogenous gibberellins inhibit coffee (Coffea arabica cv. Rubi) seed germination and cause cell death in the embryo
J. Exp. Bot.,
March 1, 2005;
56(413):
1029 - 1038.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-Y. Han, N. Kitahata, K. Sekimata, T. Saito, M. Kobayashi, K. Nakashima, K. Yamaguchi-Shinozaki, K. Shinozaki, S. Yoshida, and T. Asami
A Novel Inhibitor of 9-cis-Epoxycarotenoid Dioxygenase in Abscisic Acid Biosynthesis in Higher Plants
Plant Physiology,
July 1, 2004;
135(3):
1574 - 1582.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ogawa, A. Hanada, Y. Yamauchi, A. Kuwahara, Y. Kamiya, and S. Yamaguchi
Gibberellin Biosynthesis and Response during Arabidopsis Seed Germination
PLANT CELL,
July 1, 2003;
15(7):
1591 - 1604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Borisjuk, H. Rolletschek, U. Wobus, and H. Weber
Differentiation of legume cotyledons as related to metabolic gradients and assimilate transport into seeds
J. Exp. Bot.,
January 3, 2003;
54(382):
503 - 512.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Cosgrove, L. C. Li, H.-T. Cho, S. Hoffmann-Benning, R. C. Moore, and D. Blecker
The Growing World of Expansins
Plant Cell Physiol.,
December 15, 2002;
43(12):
1436 - 1444.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Chen, H. Nonogaki, and K. J. Bradford
A gibberellin-regulated xyloglucan endotransglycosylase gene is expressed in the endosperm cap during tomato seed germination
J. Exp. Bot.,
February 1, 2002;
53(367):
215 - 223.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
