|
Plant Physiol, November 2001, Vol. 127, pp. 949-962
Molecular Characterization of Two Arabidopsis Ire1 Homologs,
Endoplasmic Reticulum-Located Transmembrane Protein
Kinases1
Nozomu
Koizumi,
Immaculada M.
Martinez,
Yukio
Kimata,
Kenji
Kohno,
Hiroshi
Sano, and
Maarten J.
Chrispeels*
Division of Biology, University of California San Diego, La Jolla,
California 92039-0116 (N.K., I.M.M., M.J.C.); and Research and
Education Center for Genetic Information, Nara Institute of Science and
Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan (Y.K., K.K.,
H.S.)
A major response of eukaryotic cells to the presence of unfolded
proteins in the lumen of the endoplasmic reticulum (ER) is to activate
genes that encode ER-located molecular chaperones, such as the binding
protein. This response, called the unfolded protein response, requires
the transduction of a signal from the ER to the nucleus. In yeast
(Saccharomyces cerevisiae) and mammalian cells, an
ER-located transmembrane receptor protein kinase/ribonuclease called
Ire1, with a sensor domain in the lumen of the ER, is the first
component of this pathway. Here, we report the cloning and derived
amino acid sequences of AtIre1-1 and AtIre1-2, two Arabidopsis homologs
of Ire1. The two proteins are located in the perinuclear ER (based on
heterologous expression of fusions with green fluorescent protein). The
expression patterns of the two genes (using  -glucuronidase fusions)
are nearly nonoverlapping. We also demonstrate functional complementation of the sensor domains of the two proteins in yeast and
show that the Ire1-2 protein is capable of autotransphosphorylation. These and other findings are discussed in relation to the involvement of these genes in unfolded protein response signaling in plants.
1
This work has been supported by a grant from the
Department of Energy (Office of Energy Biosciences, grant no.
DE-FG03-86ER13497) to M.J.C., a fellowship from the Ministry of
Science and Technology of Spain to I.M.M., and grants from the Research
for the Future Program (JSPS-RFTF00L01604) of the Japan Society for
the Promotion of Science.
*
Corresponding author; e-mail mchrispeels{at}ucsd.edu; fax
858-534-4052.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
M. D. L. Costa, P. A. B. Reis, M. A. S. Valente, A. S. T. Irsigler, C. M. Carvalho, M. E. Loureiro, F. J. L. Aragao, R. S. Boston, L. G. Fietto, and E. P. B. Fontes
A New Branch of Endoplasmic Reticulum Stress Signaling and the Osmotic Signal Converge on Plant-specific Asparagine-rich Proteins to Promote Cell Death
J. Biol. Chem.,
July 18, 2008;
283(29):
20209 - 20219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Alandete-Saez, M. Ron, and S. McCormick
GEX3, Expressed in the Male Gametophyte and in the Egg Cell of Arabidopsis thaliana, Is Essential for Micropylar Pollen Tube Guidance and Plays a Role during Early Embryogenesis
Mol Plant,
July 1, 2008;
1(4):
586 - 598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Watanabe and E. Lam
BAX Inhibitor-1 Modulates Endoplasmic Reticulum Stress-mediated Programmed Cell Death in Arabidopsis
J. Biol. Chem.,
February 8, 2008;
283(6):
3200 - 3210.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-X. Liu, R. Srivastava, P. Che, and S. H. Howell
An Endoplasmic Reticulum Stress Response in Arabidopsis Is Mediated by Proteolytic Processing and Nuclear Relocation of a Membrane-Associated Transcription Factor, bZIP28
PLANT CELL,
December 1, 2007;
19(12):
4111 - 4119.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Wang, S. Narendra, and N. Fedoroff
Heterotrimeric G protein signaling in the Arabidopsis unfolded protein response
PNAS,
March 6, 2007;
104(10):
3817 - 3822.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. K. Johnston, N. P. Jacob, and M. R. Brodl
Heat Shock-Induced Changes in Lipid and Protein Metabolism in the Endoplasmic Reticulum of Barley Aleurone Layers
Plant Cell Physiol.,
January 1, 2007;
48(1):
31 - 41.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Iwata and N. Koizumi
An Arabidopsis transcription factor, AtbZIP60, regulates the endoplasmic reticulum stress response in a manner unique to plants
PNAS,
April 5, 2005;
102(14):
5280 - 5285.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kimata, D. Oikawa, Y. Shimizu, Y. Ishiwata-Kimata, and K. Kohno
A role for BiP as an adjustor for the endoplasmic reticulum stress-sensing protein Ire1
J. Cell Biol.,
November 8, 2004;
167(3):
445 - 456.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Koiwa, F. Li, M. G. McCully, I. Mendoza, N. Koizumi, Y. Manabe, Y. Nakagawa, J. Zhu, A. Rus, J. M. Pardo, et al.
The STT3a Subunit Isoform of the Arabidopsis Oligosaccharyltransferase Controls Adaptive Responses to Salt/Osmotic Stress
PLANT CELL,
October 1, 2003;
15(10):
2273 - 2284.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Wang, J. F. Harper, and M. Gribskov
Systematic Trans-Genomic Comparison of Protein Kinases between Arabidopsis and Saccharomyces cerevisiae
Plant Physiology,
August 1, 2003;
132(4):
2152 - 2165.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. M. Martinez and M. J. Chrispeels
Genomic Analysis of the Unfolded Protein Response in Arabidopsis Shows Its Connection to Important Cellular Processes
PLANT CELL,
February 1, 2003;
15(2):
561 - 576.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-F. Chen, M. D. Randlett, J. L. Findell, and G. E. Schaller
Localization of the Ethylene Receptor ETR1 to the Endoplasmic Reticulum of Arabidopsis
J. Biol. Chem.,
May 24, 2002;
277(22):
19861 - 19866.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Okushima, N. Koizumi, Y. Yamaguchi, Y. Kimata, K. Kohno, and H. Sano
Isolation and Characterization of a Putative Transducer of Endoplasmic Reticulum Stress in Oryza sativa
Plant Cell Physiol.,
May 15, 2002;
43(5):
532 - 539.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
