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Plant Physiol, November 2001, Vol. 127, pp. 986-997
Dissection of the Light Signal Transduction Pathways Regulating
the Two Early Light-Induced Protein Genes in
Arabidopsis
Orit
Harari-Steinberg,
Itzhak
Ohad, and
Daniel A.
Chamovitz*
Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel (O.H.-S., D.A.C.); and Department of Biological Chemistry, The
Hebrew University of Jerusalem, Jerusalem, Israel (I.O.)
The expression of light-regulated genes in plants is
controlled by different classes of photoreceptors that act through a variety of signaling molecules. During photomorphogenesis, the early
light-induced protein (Elip) genes are among the first
to be induced. To understand the light signal transduction pathways that regulate Elip expression, the two
Elip genes, Elip1 and
Elip2, in Arabidopsis were studied, taking advantage of
the genetic tools available for studying light signaling in
Arabidopsis. Using two independent quantitative reverse
transcriptase-PCR techniques, we found that red, far-red, and blue
lights positively regulate expression of the Elip genes.
Phytochrome A and phytochrome B are involved in this signaling. The
cryptochrome or phototropin photoreceptors are not required for
blue-light induction of either Elip gene, suggesting the
involvement of an additional, unidentified, blue-light receptor.
Although the COP9 signalosome, a downstream regulator, is involved in
dark repression of both Elips, Elip1 and
Elip2 show different expression patterns in the dark.
The transcription factor HY5 promotes the light induction of
Elip1, but not Elip2. A defect in
photosystem II activity in greening of hy5 seedlings may
result from the loss of Elip1. Heat shock positively
controlled Elip1 and Elip2 in a
light-independent fashion. This induction is independent of
HY5, indicating that heat shock and light activate
transcription of the Elip genes through independent pathways.
*
Corresponding author; e-mail dannyc{at}tauex.tau.ac.il; fax 97236409380.
© 2001 American Society of Plant Physiologists
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