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Plant Physiol, December 2001, Vol. 127, pp. 1466-1475

UPDATE
Signal Transduction in Maize and Arabidopsis Mesophyll Protoplasts1

Jen Sheen*

Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Wellman 11, 50 Blossom Street, Boston, Massachusetts 02114

Plant protoplasts show physiological perceptions and responses to hormones, metabolites, environmental cues, and pathogen-derived elicitors, similar to cell-autonomous responses in intact tissues and plants. The development of defined protoplast transient expression systems for high-throughput screening and systematic characterization of gene functions has greatly contributed to elucidating plant signal transduction pathways, in combination with genetic, genomic, and transgenic approaches.


1 This work was supported by the National Science Foundation, by the U.S. Department of Agriculture, by the National Institutes of Health, and by Hoechst A.G.

* E-mail sheen{at}molbio.mgh.harvard.edu; fax 617-726-6893.

© 2001 American Society of Plant Physiologists



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