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Plant Physiol, December 2001, Vol. 127, pp. 1556-1567
An Aquaglyceroporin Is Abundantly Expressed Early in the
Development of the Suspensor and the Embryo Proper of Loblolly
Pine1
Vincent T.
Ciavatta,
Raphael
Morillon,
Gerald S.
Pullman,
Maarten
J.
Chrispeels, and
John
Cairney*
Institute of Paper Science and Technology, Forest Biology Group,
500 10th Street, Atlanta, Georgia 30318 (V.T.C., G.S.P., J.C.); and
Division of Biology, University of California, San Diego, California
92093-0116 (R.M., M.J.C.)
In contrast to angiosperms, pines and other gymnosperms form
well-developed suspensors in somatic embryogenic cultures. This creates
a useful system to study suspensor biology. In a study of gene
expression during the early stages of conifer embryogenesis, we
identified a transcript, PtNIP1;1, that is abundant in
immature loblolly pine (Pinus taeda) zygotic and somatic
embryos, but is undetectable in later-stage embryos, megagametophytes,
and roots, stems, and needles from 1 year-old seedlings. Analysis of
PtNIP1;1 transcript in embryo proper and suspensor
tissues by reverse transcription-polymerase chain reaction suggests
preferential expression in the suspensor. Based on comparisons of
derived amino acid sequences, PtNIP1;1 belongs to the nodulin-like
members of the major intrinsic protein superfamily branch of the
aquaporin (major intrinsic protein) superfamily. Through heterologous
expression in Xenopus laevis oocytes and the yeast
(Saccharomyces cerevisiae)
fps1 mutant, PtNIP1;1 has been shown
to be an active aquaglyceroporin.
1
This work was supported by the Member Companies
of Institute of Paper Science and Technology (studentship to
V.T.C.).
*
Corresponding author; e-mail john.cairney{at}ipst.edu; fax
404-894-4778.
© 2001 American Society of Plant Physiologists
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