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Plant Physiol, December 2001, Vol. 127, pp. 1579-1589
A Physical Amplified Fragment-Length Polymorphism Map of
Arabidopsis
Janny L.
Peters,1*
Hans
Constandt,
Pia
Neyt,
Gerda
Cnops,
Jan
Zethof,
Marc
Zabeau, and
Tom
Gerats1
Flanders Interuniversity Institute for Biotechnology, University of
Ghent, Department of Plant Genetics, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium
We have positioned amplified fragment-length polymorphism (AFLP)
markers directly on the genome sequence of a complex organism, Arabidopsis, by combining gel-based AFLP analysis with in silico restriction fragment analysis using the published genome sequence. For
placement of the markers, we used information on restriction fragment size, four selective nucleotides, and the rough genetic position of the markers as deduced from the analysis of a limited number of Columbia (Col)/Landsberg (Ler) recombinant
inbred lines. This approach allows for exact physical positioning of
markers as opposed to the statistical localization resulting from
traditional genetic mapping procedures. In addition, it is fast because
no extensive segregation analysis is needed. In principle, the method can be applied to all organisms for which a complete or nearly complete
genome sequence is available. We have located 1,267 AFLP Col/Ler markers resulting from 256 SacI+2, MseI+2 primer combinations to a
physical position on the Arabidopsis genome. The positioning was
verified by sequence analysis of 70 markers and by segregation analysis
of two leaf-form mutants. Approximately 50% of the mapped Col/Ler AFLP markers can be used for segregation
analysis in Col/C24, Col/Wassilewskija, or Col/Cape Verde Islands
crosses. We present data on one such cross: the localization of a
viviparous-like mutant segregating in a Col/C24 cross.
1
Present address: Dept. of Experimental Botany,
University of Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands.
*
Corresponding author; e-mail japet{at}sci.kun.nl; fax
32-24-3652787.
© 2001 American Society of Plant Physiologists
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