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Plant Physiol, December 2001, Vol. 127, pp. 1595-1606
Characterization of a Family of Arabidopsis Genes Related to
Xyloglucan Fucosyltransferase11
Rodrigo
Sarria,2
Tanya A.
Wagner,
Malcolm A.
O'Neill,
Ahmed
Faik,
Curtis G.
Wilkerson,
Kenneth
Keegstra, and
Natasha V.
Raikhel*
Michigan State University-Department of Energy Plant
Research Laboratory (R.S., T.A.W., A.F., C.G.W., K.K., N.V.R.),
Departments of Plant Biology (K.K.) and Biochemistry and Molecular
Biology (K.K., N.V.R.), Michigan State University, East Lansing,
Michigan 48824; and Complex Carbohydrate Research Center, University of
Georgia, 220 Riverbend Road, Athens, Georgia 30602 (M.A.O.)
To understand primary cell wall assembly in Arabidopsis, we
have focused on identifying and characterizing enzymes involved in
xyloglucan biosynthesis. Nine genes (AtFUT2-10) were
identified that share between 47% and 62% amino acid similarity with
the xyloglucan-specific fucosyltransferase AtFUT1.
Reverse transcriptase-PCR analysis indicates that all these
genes are expressed. Bioinformatic analysis predicts that these family
members are fucosyltransferases, and we first hypothesized that some
may also be involved in xyloglucan biosynthesis. AtFUT3,
AtFUT4, and AtFUT5 were expressed in
tobacco (Nicotiana tabacum L. cv BY2) suspension culture
cells, and the resulting proteins did not transfer fucose (Fuc) from
GDP-Fuc to tamarind xyloglucan. AtFUT3, AtFUT4, and AtFUT5 were
overexpressed in Arabidopsis plants. Leaves of plants overexpressing
AtFUT4 or AtFUT5 contained more Fuc than wild-type plants. Stems of
plants overexpressing AtFUT4 or AtFUT5 contained more xylose, less
arabinose, and less galactose than wild-type plants. We suggest that
the AtFUT family is likely to include fucosyltransferases important for
the synthesis of wall carbohydrates. A targeted analysis of isolated
cell wall matrix components from plants altered in expression of these
proteins will help determine their specificity and biological function.
1
This work was supported by the National Science
Foundation Plant Genome Program (grant no. DBI-9975815), by the
Department of Energy Biosciences program (grant no. DE-FG0201ER
to K.K. and N.V.R.), and by the Department of Energy (grant nos.
DE-FG05-93ER20115 and DE-FG09-93ER20097 to M.A.O.).
2
Present address: BASF Plant Science, L.L.C., 26 Davis
Drive, Research Triangle Park, NC 27709.
*
Corresponding author; e-mail nraikhel{at}msu.edu; fax
517-353-9168.
© 2001 American Society of Plant Physiologists
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