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Plant Physiol, December 2001, Vol. 127, pp. 1644-1655

A Chloroplast Protein Homologous to the Eubacterial Topological Specificity Factor MinE Plays a Role in Chloroplast Division1

Ryuuichi Itoh,2* Makoto Fujiwara,2 Noriko Nagata, and Shigeo Yoshida

Plant Functions Laboratory (R.I., M.F., S.Y.) and Plant Science Center (N.N., S.Y.), RIKEN, Wako, Saitama 351-0198, Japan

We report the identification of a nucleus-encoded minE gene, designated AtMinE1, of Arabidopsis. The encoded AtMinE1 protein possesses both N- and C-terminal extensions, relative to the eubacterial and algal chloroplast-encoded MinE proteins. The N-terminal extension functioned as a chloroplast-targeting transit peptide, as revealed by a transient expression assay using an N terminus:green fluorescent protein fusion. Histochemical beta -glucuronidase staining of transgenic Arabidopsis lines harboring an AtMinE1 promoter::uidA reporter fusion unveiled specific activation of the promoter in green tissues, especially at the shoot apex, which suggests a requirement for cell division-associated AtMinE1 expression for proplastid division in green tissues. In addition, we generated transgenic plants overexpressing a full-length AtMinE1 cDNA and examined the subcellular structures of those plants. Giant heteromorphic chloroplasts were observed in transgenic plants, with a reduced number per cell, whereas mitochondrial morphology remained similar to that of wild-type plants. Taken together, these observations suggest that MinE is the third conserved component involved in chloroplast division.


1 This work was supported by the Ministry of Education, Culture, Sports, Science and Technology of Japan (Grant-in-Aid no. 12740452 to R.I.), by the President's Special Research Grant from RIKEN (grant to R.I.), and by the Special Postdoctoral Researcher's Program of RIKEN (R.I., M.F., and N.N.).

2 These authors contributed equally to the paper.

* Corresponding author; e-mail: ryuitoh{at}postman.riken.go.jp; fax 81-48-462-4674.

© 2001 American Society of Plant Physiologists



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