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Plant Physiol, December 2001, Vol. 127, pp. 1644-1655
A Chloroplast Protein Homologous to the Eubacterial Topological
Specificity Factor MinE Plays a Role in Chloroplast
Division1
Ryuuichi
Itoh,2*
Makoto
Fujiwara,2
Noriko
Nagata, and
Shigeo
Yoshida
Plant Functions Laboratory (R.I., M.F., S.Y.) and Plant Science
Center (N.N., S.Y.), RIKEN, Wako, Saitama 351-0198, Japan
We report the identification of a nucleus-encoded
minE gene, designated AtMinE1, of
Arabidopsis. The encoded AtMinE1 protein possesses both N- and
C-terminal extensions, relative to the eubacterial and algal
chloroplast-encoded MinE proteins. The N-terminal extension functioned
as a chloroplast-targeting transit peptide, as revealed by a transient
expression assay using an N terminus:green fluorescent protein fusion.
Histochemical  -glucuronidase staining of transgenic Arabidopsis
lines harboring an AtMinE1
promoter::uidA reporter fusion unveiled
specific activation of the promoter in green tissues, especially at the
shoot apex, which suggests a requirement for cell division-associated
AtMinE1 expression for proplastid division in green
tissues. In addition, we generated transgenic plants overexpressing a
full-length AtMinE1 cDNA and examined the subcellular structures of those plants. Giant heteromorphic chloroplasts were observed in transgenic plants, with a reduced number per cell, whereas
mitochondrial morphology remained similar to that of wild-type plants.
Taken together, these observations suggest that MinE is the third
conserved component involved in chloroplast division.
1
This work was supported by the Ministry of
Education, Culture, Sports, Science and Technology of Japan
(Grant-in-Aid no. 12740452 to R.I.), by the President's Special
Research Grant from RIKEN (grant to R.I.), and by the Special
Postdoctoral Researcher's Program of RIKEN (R.I., M.F., and N.N.).
2
These authors contributed equally to the paper.
*
Corresponding author; e-mail: ryuitoh{at}postman.riken.go.jp; fax
81-48-462-4674.
© 2001 American Society of Plant Physiologists
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