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Plant Physiol, December 2001, Vol. 127, pp. 1667-1675
Arabidopsis RTM1 and RTM2 Genes Function
in Phloem to Restrict Long-Distance Movement of Tobacco Etch
Virus1
Stephen T.
Chisholm,2
Michael A.
Parra,
Robert J.
Anderberg, and
James C.
Carrington3*
Institute of Biological Chemistry, Washington State University,
Pullman, Washington 99164-6340
Restriction of long-distance movement of tobacco etch virus (TEV)
in Arabidopsis ecotype Col-0 plants requires the function of at least
three genes: RTM1 (restricted TEV movement 1),
RTM2, and RTM3. The mechanism of TEV
movement restriction remains poorly understood, although it does not
involve a hypersensitive response or systemic acquired resistance. A
functional characterization of RTM1 and
RTM2 was done. The RTM1 protein was found to be soluble with the potential to form self-interacting complexes. The regulatory regions of both the RTM1 and RTM2 genes
were analyzed using reporter constructs. The regulatory sequences from
both genes directed expression of  -glucuronidase exclusively in
phloem-associated cells. Translational fusion proteins containing the
green fluorescent protein and RTM1 or RTM2 localized to sieve elements
when expressed from their native regulatory sequences. Thus, components
of the RTM system may function within phloem, and sieve elements in
particular, to restrict TEV long-distance movement.
1
This work was supported by the National
Institutes of Health (grant nos. AI43288 and AI27832) and by the U.S.
Department of Agriculture (grant no. NRI 98-35303-6485).
2
Present address: Department of Plant and Microbial
Biology, University of California, Berkeley, CA 94720-3102.
3
Present address: Center for Gene Research and
Biotechnology, Oregon State University, Corvallis, OR
97331-7303.
*
Corresponding author; e-mail carrington{at}orst.edu; fax
541-737-3045.
© 2001 American Society of Plant Physiologists
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