|
Plant Physiol, December 2001, Vol. 127, pp. 1682-1693
GAMYB-like Genes, Flowering, and Gibberellin
Signaling in Arabidopsis1
Gregory F.W.
Gocal,23
Candice C.
Sheldon,2
Frank
Gubler,
Thomas
Moritz,
David J.
Bagnall,
Colleen P.
MacMillan,
Song F.
Li,
Roger W.
Parish,
Elizabeth S.
Dennis,
Detlef
Weigel,4 and
Rod W.
King*
Commonwealth Scientific and Industrial Research
Organization, Plant Industry, G.P.O. Box 1600, Canberra, Australian
Capital Territory 2601, Australia (G.F.W.G., C.C.S., F.G., D.J.B.,
C.P.M., E.S.D., R.W.K.); Plant Biology Laboratory, The Salk Institute
for Biological Studies, 10010 North Torrey Pines Road, La Jolla,
California 92037 (G.F.W.G., D.W.); Swedish University of Agricultural
Sciences, Umeå Plant Science Centre, S-901 83 Umeå, Sweden (T.M.);
and School of Botany, La Trobe University, Melbourne, Victoria,
Australia (S.F.L., R.W.P.)
We have identified three Arabidopsis genes with
GAMYB-like activity, AtMYB33,
AtMYB65, and AtMYB101, which can
substitute for barley (Hordeum vulgare) GAMYB in
transactivating the barley  -amylase promoter. We have investigated
the relationships between gibberellins (GAs), these
GAMYB-like genes, and petiole elongation and flowering
of Arabidopsis. Within 1 to 2 d of transferring plants from short-
to long-day photoperiods, growth rate and erectness of petioles
increased, and there were morphological changes at the shoot apex
associated with the transition to flowering. These responses were
accompanied by accumulation of GAs in the petioles (GA1 by
11-fold and GA4 by 3-fold), and an increase in expression of AtMYB33 at the shoot apex. Inhibition of GA
biosynthesis using paclobutrazol blocked the petiole elongation induced
by long days. Causality was suggested by the finding that, with GA
treatment, plants flowered in short days, AtMYB33
expression increased at the shoot apex, and the petioles elongated and
grew erect. That AtMYB33 may mediate a GA signaling role in flowering
was supported by its ability to bind to a specific 8-bp sequence in the
promoter of the floral meristem-identity gene, LEAFY,
this same sequence being important in the GA response of the
LEAFY promoter. One or more of these
AtMYB genes may also play a role in the root tip during
germination and, later, in stem tissue. These findings extend our
earlier studies of GA signaling in the Gramineae to include a dicot
species, Arabidopsis, and indicate that GAMYB-like genes
may mediate GA signaling in growth and flowering responses.
1
This work was supported by the Commonwealth
Scientific and Industrial Research Organization (to G.F.W.G) and by the
Australian Research Council (to S.L. and R.W.P.), the Human Frontiers
Science Program Organization (to T.M. and D.W.), and the National
Science Foundation (to D.W.). C.C.S. was supported in part by the Ken and Yasuko Myer Plant Science Research Fund.
2
These authors contributed equally to the paper.
3
Present address: Valigen, Plant and Industrial Products,
11180 Roselle St., San Diego, CA 92121.
4
Present address: Department of Molecular Biology, Max
Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
*
Corresponding author: e-mail r.king{at}csiro.au; fax
61-262-465-000.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
E. Mutasa-Gottgens and P. Hedden
Gibberellin as a factor in floral regulatory networks
J. Exp. Bot.,
May 1, 2009;
60(7):
1979 - 1989.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Wilkins, H. Nahal, J. Foong, N. J. Provart, and M. M. Campbell
Expansion and Diversification of the Populus R2R3-MYB Family of Transcription Factors
Plant Physiology,
February 1, 2009;
149(2):
981 - 993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. N. Devaiah, R. Madhuvanthi, A. S. Karthikeyan, and K. G. Raghothama
Phosphate Starvation Responses and Gibberellic Acid Biosynthesis Are Regulated by the MYB62 Transcription Factor in Arabidopsis
Mol Plant,
January 1, 2009;
2(1):
43 - 58.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hisamatsu and R. W. King
The nature of floral signals in Arabidopsis. II. Roles for FLOWERING LOCUS T (FT) and gibberellin
J. Exp. Bot.,
October 17, 2008;
(2008)
ern232v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. W. King, T. Hisamatsu, E. E. Goldschmidt, and C. Blundell
The nature of floral signals in Arabidopsis. I. Photosynthesis and a far-red photoresponse independently regulate flowering by increasing expression of FLOWERING LOCUS T (FT)
J. Exp. Bot.,
October 3, 2008;
(2008)
ern231v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. W. King, L. N. Mander, T. Asp, C. P. MacMillan, C. A. Blundell, and L. T. Evans
Selective Deactivation of Gibberellins below the Shoot Apex is Critical to Flowering but Not to Stem Elongation of Lolium
Mol Plant,
March 1, 2008;
1(2):
295 - 307.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Bai and D. A. DeMason
Hormone interactions and regulation of PsPK2::GUS compared with DR5::GUS and PID::GUS in Arabidopsis thaliana
Am. J. Botany,
February 1, 2008;
95(2):
133 - 145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Alves-Ferreira, F. Wellmer, A. Banhara, V. Kumar, J. L. Riechmann, and E. M. Meyerowitz
Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development
Plant Physiology,
November 1, 2007;
145(3):
747 - 762.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Zentella, Z.-L. Zhang, M. Park, S. G. Thomas, A. Endo, K. Murase, C. M. Fleet, Y. Jikumaru, E. Nambara, Y. Kamiya, et al.
Global Analysis of DELLA Direct Targets in Early Gibberellin Signaling in Arabidopsis
PLANT CELL,
October 1, 2007;
19(10):
3037 - 3057.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Gallego-Giraldo, J. L. Garcia-Martinez, T. Moritz, and I. Lopez-Diaz
Flowering in Tobacco Needs Gibberellins but is not Promoted by the Levels of Active GA1 and GA4 in the Apical Shoot
Plant Cell Physiol.,
June 1, 2007;
48(6):
897 - 898.
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Gallego-Giraldo, J. L. Garcia-Martinez, T. Moritz, and I. Lopez-Diaz
Flowering in Tobacco Needs Gibberellins but is not Promoted by the Levels of Active GA1 and GA4 in the Apical Shoot
Plant Cell Physiol.,
April 1, 2007;
48(4):
615 - 625.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Penfield, Y. Li, A. D. Gilday, S. Graham, and I. A. Graham
Arabidopsis ABA INSENSITIVE4 Regulates Lipid Mobilization in the Embryo and Reveals Repression of Seed Germination by the Endosperm
PLANT CELL,
August 1, 2006;
18(8):
1887 - 1899.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Bai and D. A. DeMason
Hormone Interactions and Regulation of Unifoliata, PsPK2, PsPIN1 and LE Gene Expression in Pea (Pisum sativum) Shoot Tips
Plant Cell Physiol.,
July 1, 2006;
47(7):
935 - 948.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. W. King, T. Moritz, L. T. Evans, J. Martin, C. H. Andersen, C. Blundell, I. Kardailsky, and P. M. Chandler
Regulation of Flowering in the Long-Day Grass Lolium temulentum by Gibberellins and the FLOWERING LOCUS T Gene
Plant Physiology,
June 1, 2006;
141(2):
498 - 507.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P. MacMillan, C. A. Blundell, and R. W. King
Flowering of the Grass Lolium perenne. Effects of Vernalization and Long Days on Gibberellin Biosynthesis and Signaling
Plant Physiology,
July 1, 2005;
138(3):
1794 - 1806.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hisamatsu, R. W. King, C. A. Helliwell, and M. Koshioka
The Involvement of Gibberellin 20-Oxidase Genes in Phytochrome-Regulated Petiole Elongation of Arabidopsis
Plant Physiology,
June 1, 2005;
138(2):
1106 - 1116.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. Millar and F. Gubler
The Arabidopsis GAMYB-Like Genes, MYB33 and MYB65, Are MicroRNA-Regulated Genes That Redundantly Facilitate Anther Development
PLANT CELL,
March 1, 2005;
17(3):
705 - 721.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Champagne and N. Sinha
Compound leaves: equal to the sum of their parts?
Development,
September 15, 2004;
131(18):
4401 - 4412.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Achard, A. Herr, D. C. Baulcombe, and N. P. Harberd
Modulation of floral development by a gibberellin-regulated microRNA
Development,
July 15, 2004;
131(14):
3357 - 3365.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. G. Thomas and T.-p. Sun
Update on Gibberellin Signaling. A Tale of the Tall and the Short
Plant Physiology,
June 1, 2004;
135(2):
668 - 676.
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. K. Boss, R. M. Bastow, J. S. Mylne, and C. Dean
Multiple Pathways in the Decision to Flower: Enabling, Promoting, and Resetting
PLANT CELL,
June 1, 2004;
16(suppl_1):
S18 - S31.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Shikata, Y. Matsuda, K. Ando, A. Nishii, M. Takemura, A. Yokota, and T. Kohchi
Characterization of Arabidopsis ZIM, a member of a novel plant-specific GATA factor gene family
J. Exp. Bot.,
March 1, 2004;
55(397):
631 - 639.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-H. Han, S. Goud, L. Song, and N. Fedoroff
The Arabidopsis double-stranded RNA-binding protein HYL1 plays a role in microRNA-mediated gene regulation
PNAS,
January 27, 2004;
101(4):
1093 - 1098.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kaneko, Y. Inukai, M. Ueguchi-Tanaka, H. Itoh, T. Izawa, Y. Kobayashi, T. Hattori, A. Miyao, H. Hirochika, M. Ashikari, et al.
Loss-of-Function Mutations of the Rice GAMYB Gene Impair {alpha}-Amylase Expression in Aleurone and Flower Development
PLANT CELL,
January 1, 2004;
16(1):
33 - 44.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Washio
Functional Dissections between GAMYB and Dof Transcription Factors Suggest a Role for Protein-Protein Associations in the Gibberellin-Mediated Expression of the RAmy1A Gene in the Rice Aleurone
Plant Physiology,
October 1, 2003;
133(2):
850 - 863.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. JACQMARD, I. GADISSEUR, and G. BERNIER
Cell Division and Morphological Changes in the Shoot Apex of Arabidopsis thaliana during Floral Transition
Ann. Bot.,
April 1, 2003;
91(5):
571 - 576.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Blazquez, M. Trenor, and D. Weigel
Independent Control of Gibberellin Biosynthesis and Flowering Time by the Circadian Clock in Arabidopsis
Plant Physiology,
December 1, 2002;
130(4):
1770 - 1775.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Olszewski, T.-p. Sun, and F. Gubler
Gibberellin Signaling: Biosynthesis, Catabolism, and Response Pathways
PLANT CELL,
May 1, 2002;
14(90001):
S61 - 80.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Mouradov, F. Cremer, and G. Coupland
Control of Flowering Time: Interacting Pathways as a Basis for Diversity
PLANT CELL,
May 1, 2002;
14(90001):
S111 - 130.
[Full Text]
[PDF]
|
|
|
|
|
